GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:2117276
| Citation |
Weickert, MJ and Chambliss, GH (1990) Site-directed mutagenesis of a catabolite repression operator sequence in Bacillus subtilis. Proc. Natl. Acad. Sci. U.S.A. 87:6238-42 |
|---|---|
| Abstract |
Catabolite repression of the Bacillus subtilis alpha-amylase gene (amyE) involves an operator sequence located just downstream of the promoter (amyR), overlapping the transcription start site. Oligonucleotide site-directed mutagenesis of this sequence identified bases required for catabolite repression. Two mutations increased both the 2-fold symmetry of the operator and the repression ratio. Although many mutations reduced the repression ratio 3- to 11-fold, some also caused a 2-fold or greater increase in amylase production. Others caused hyperproduction without affecting catabolite repression. Homologous sequences in other catabolite-repressed B. subtilis promoters suggest a common regulatory site may be involved in catabolite repression. |
| Links | |
| Keywords |
Bacillus subtilis/genetics; Base Sequence; Cloning, Molecular; DNA, Bacterial/genetics; Enzyme Repression; Genes, Bacterial; Molecular Sequence Data; Mutation; Oligonucleotide Probes; Operon; Plasmids; Promoter Regions, Genetic; Restriction Mapping; Sequence Homology, Nucleic Acid; alpha-Amylases/biosynthesis; alpha-Amylases/genetics |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
See also
References
See Help:References for how to manage references in GONUTS.
