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PMID:21103397
| Citation |
Krichevsky, A, Meyers, B, Vainstein, A, Maliga, P and Citovsky, V (2010) Autoluminescent plants. PLoS ONE 5:e15461 |
|---|---|
| Abstract |
Prospects of obtaining plants glowing in the dark have captivated the imagination of scientists and layman alike. While light emission has been developed into a useful marker of gene expression, bioluminescence in plants remained dependent on externally supplied substrate. Evolutionary conservation of the prokaryotic gene expression machinery enabled expression of the six genes of the lux operon in chloroplasts yielding plants that are capable of autonomous light emission. This work demonstrates that complex metabolic pathways of prokaryotes can be reconstructed and function in plant chloroplasts and that transplastomic plants can emit light that is visible by naked eye. |
| Links |
PubMed PMC2980496 Online version:10.1371/journal.pone.0015461 |
| Keywords |
Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Chloroplasts/chemistry; Chloroplasts/genetics; Chloroplasts/metabolism; Genome, Chloroplast/genetics; Luminescence; Photobacterium/genetics; Photobacterium/metabolism; Plants, Genetically Modified; Tobacco/chemistry; Tobacco/genetics; Tobacco/metabolism; Transcription Factors/chemistry; Transcription Factors/genetics; Transcription Factors/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0008218: bioluminescence |
ECO:0000315: |
P |
Amplified luxB and luxC to further confirm the presence of the lux operon within the transplastomic genome (Fig. 1B). Figure 2 DNA gel blot analysis confirms plastid transformation with vectors pCAS3-LUX-rps12/TrnV (A) and pCAS3-LUX-trnI/trnA (B). Enzymes involved in the synthesis of aldehyde substrate utilized in the light emission reaction |
complete | ||||
| GO:0008218: bioluminescence |
ECO:0000315: |
P |
Amplified luxB and luxC to further confirm the presence of the lux operon within the transplastomic genome (Fig. 1B). Transplastomic plants transformed with the pCAS3-LUX-TrnI/TrnA plasmid were identified in a similar fashion, using junction PCR primers specific for TrnI and TrnA, as well as for luxB and luxC. Integration of aadA and the lux operon genes in the LUX-TrnI/TrnA genome, and the absence of non-transformed wild-type ptDNA copies has been confirmed as described for the LUX-rps12/TrnV plants (Figs. 2 and 3). |
complete | ||||
See also
References
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