PMID:21103375

Citation	Fourrage, C, Chevalier, S and Houliston, E (2010) A highly conserved Poc1 protein characterized in embryos of the hydrozoan Clytia hemisphaerica: localization and functional studies. PLoS ONE 5:e13994
Abstract	Poc1 (Protein of Centriole 1) proteins are highly conserved WD40 domain-containing centriole components, well characterized in the alga Chlamydomonas, the ciliated protazoan Tetrahymena, the insect Drosophila and in vertebrate cells including Xenopus and zebrafish embryos. Functions and localizations related to the centriole and ciliary axoneme have been demonstrated for Poc1 in a range of species. The vertebrate Poc1 protein has also been reported to show an additional association with mitochondria, including enrichment in the specialized "germ plasm" region of Xenopus oocytes. We have identified and characterized a highly conserved Poc1 protein in the cnidarian Clytia hemisphaerica. Clytia Poc1 mRNA was found to be strongly expressed in eggs and early embryos, showing a punctate perinuclear localization in young oocytes. Fluorescence-tagged Poc1 proteins expressed in developing embryos showed strong localization to centrioles, including basal bodies. Anti-human Poc1 antibodies decorated mitochondria in Clytia, as reported in human cells, but failed to recognise endogenous or fluorescent-tagged Clytia Poc1. Injection of specific morpholino oligonucleotides into Clytia eggs prior to fertilization to repress Poc1 mRNA translation interfered with cell division from the blastula stage, likely corresponding to when neosynthesis normally takes over from maternally supplied protein. Cell cycle lengthening and arrest were observed, phenotypes consistent with an impaired centriolar biogenesis or function. The specificity of the defects could be demonstrated by injection of synthetic Poc1 mRNA, which restored normal development. We conclude that in Clytia embryos, Poc1 has an essentially centriolar localization and function.
Links	PubMed PMC2982836 Online version:10.1371/journal.pone.0013994
Keywords	Animals; Blotting, Western; Cell Cycle Proteins/classification; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Centrioles/metabolism; DNA, Complementary/chemistry; DNA, Complementary/genetics; Embryo, Nonmammalian/embryology; Embryo, Nonmammalian/metabolism; Female; Fluorescent Antibody Technique; Gene Expression Regulation, Developmental; Gene Library; Hydrozoa/embryology; Hydrozoa/genetics; Hydrozoa/metabolism; In Situ Hybridization; Microscopy, Confocal; Molecular Sequence Data; Oocytes/metabolism; Phylogeny; Sequence Analysis, DNA
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9CNID:D6QUQ2	part_of	GO:0036064: ciliary basal body	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
9CNID:D6QUQ2	involved_in	GO:0051781: positive regulation of cell division	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
9CNID:D6QUQ2		GO:0036064: ciliary basal body	ECO:0000314:		C		figure 3	complete CACAO 6725
9CNID:D6QUQ2		GO:0048232: male gamete generation	ECO:0000314:		P		Figure 2	complete CACAO 6726
9CNID:D6QUQ2		GO:0051781: positive regulation of cell division	ECO:0000315:		P		fig 5	complete CACAO 6728

References

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