PMID:21070858

Citation	Khatun, R and Lakin-Thomas, P (2011) Activation and localization of protein kinase C in Neurospora crassa. Fungal Genet. Biol. 48:465-73
Abstract	The Neurosporacrassa protein kinase C (NPKC) is reported to be a regulator of light responsive genes. It phosphorylates the light receptor WC-1 and regulates the levels of the circadian clock protein FRQ and transcription of the light-induced albino-2 gene. In mammals, the conventional and novel isoforms of PKC are activated by diacylglycerol (DAG), which induces PKC translocation from the cytoplasm to membranes. To investigate the interaction of NPKC and DAG in Neurospora, we constructed a strain that expresses a PKC-GFP fusion protein. We found that NPKC localizes to growing tips and sub-apical plasma membrane in actively growing hyphae, and actively participates in septum development. NPKC is activated by exogenous DAG and phorbol esters, and translocates to the plasma membrane from the cytoplasm. We have previously reported that choline depletion of the chol-1 mutant of Neurospora increases DAG levels and lengthens the period of the circadian rhythm of conidiation. We have found that the activity of NPKC is rhythmic, and that NPKC levels are increased on choline depletion. However, over-expression of NPKC did not lengthen the conidiation period, indicating that PKC in Neurospora may not be responsible for the lengthened period in low choline cultures.
Links	PubMed Online version:10.1016/j.fgb.2010.11.002
Keywords	Artificial Gene Fusion; Cell Membrane/chemistry; Cytoplasm/chemistry; Diglycerides/metabolism; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Fungal; Genes, Reporter; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Hyphae/chemistry; Neurospora crassa/metabolism; Phorbol Esters/metabolism; Protein Kinase C/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
NEUCR:KPC1	part_of	GO:0030428: cell septum	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
NEUCR:KPC1		GO:0004697: protein kinase C activity	ECO:0000314:		F		Figure 1. Neurosporacrassa protein kinase C (NPKC) activity is directly enhanced in absence of Choline. This is true for parental strain as well as for the GFP-NPKC transformants.	complete CACAO 9403
NEUCR:KPC1	NOT	GO:1900020: positive regulation of protein kinase C activity	ECO:0000314:		P		Table 2: Generally speaking, as choline concentration increases, conidiation period in neurospora crassa decreases. However, this decrease in conidiation time is not due to an increase in NPKC activity because experimental groups Q and R don't show any significant decrease in conidiation period when compared to wildtype control.	complete CACAO 9485
NEUCR:KPC1		GO:0030428: cell septum	ECO:0000314:		C		Figure 3,cross section of N.crassa septum viewed under confocal microscopy indicates that the PKC is initially localized in the cell septum.	complete CACAO 9607

References

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