PMID:20815820

Citation	Ross, JA, Wardle, SJ and Haniford, DB (2010) Tn10/IS10 transposition is downregulated at the level of transposase expression by the RNA-binding protein Hfq. Mol. Microbiol. 78:607-21
Abstract	We show in this work that disruption of the hfq gene in Escherichia coli causes a large increase in IS10 transposition when IS10 is present on a multi-copy plasmid. Hfq is an RNA-binding protein that regulates the expression of a large number of genes at the post-transcriptional level by promoting the pairing of mRNAs with partially complementary short RNAs. As the translation of IS10 transposase mRNA (RNA-IN) is inhibited by an IS10-encoded anti-sense RNA (RNA-OUT), it seemed likely that Hfq would negatively regulate Tn10/IS10 transposition by promoting anti-sense inhibition of RNA-IN translation. Consistent with this, we show that Hfq promotes pairing of RNA-IN and RNA-OUT in vitro and downregulates RNA-IN expression in vivo. However, we also show that Hfq negatively regulates Tn10 transposition when no functional anti-sense RNA is produced. Taken together, the results suggest that Hfq acts at two distinct steps to inhibit Tn10/IS10 transposition. This is the first example of Hfq regulating a bacterial transposition reaction.
Links	PubMed Online version:10.1111/j.1365-2958.2010.07359.x
Keywords	DNA Transposable Elements; Down-Regulation; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Host Factor 1 Protein/genetics; Host Factor 1 Protein/metabolism; Protein Binding; Protein Biosynthesis; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Transposases/genetics; Transposases/metabolism
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