GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:20724587
| Citation |
Broberg, CA, Zhang, L, Gonzalez, H, Laskowski-Arce, MA and Orth, K (2010) A Vibrio effector protein is an inositol phosphatase and disrupts host cell membrane integrity. Science 329:1660-2 |
|---|---|
| Abstract |
The marine bacterium Vibrio parahaemolyticus causes gastroenteritis in humans and encodes the type III effector protein VPA0450, which contributes to host cell death caused by autophagy, cell rounding, and cell lysis. We found that VPA0450 is an inositol polyphosphate 5-phosphatase that hydrolyzed the D5 phosphate from the plasma membrane phospholipid phosphatidylinositol 4,5-bisphosphate. VPA0450 disrupted cytoskeletal binding sites on the inner surface of membranes of human cells and caused plasma membrane blebbing, which compromised membrane integrity and probably contributed to cell death by facilitating lysis. Thus, bacterial pathogens can disrupt adaptor protein-binding sites required for proper membrane and cytoskeleton dynamics by altering the homeostasis of membrane-bound inositol-signaling molecules. |
| Links |
PubMed Online version:10.1126/science.1192850 |
| Keywords |
Actins/metabolism; Amino Acid Sequence; Autophagy; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Binding Sites; Cell Membrane/physiology; Cell Membrane/ultrastructure; Cell Shape; Computational Biology; Cytoskeleton/physiology; Cytoskeleton/ultrastructure; HeLa Cells; Homeostasis; Humans; Molecular Sequence Data; Phosphatidylinositol 4,5-Diphosphate/metabolism; Phosphatidylinositols/metabolism; Phosphoric Monoester Hydrolases/chemistry; Phosphoric Monoester Hydrolases/genetics; Phosphoric Monoester Hydrolases/metabolism; Protein Interaction Domains and Motifs; Signal Transduction; Transfection; Vibrio parahaemolyticus/enzymology; Vibrio parahaemolyticus/pathogenicity |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
Contributes to |
GO:0004445: inositol-polyphosphate 5-phosphatase activity |
ECO:0000315: |
F |
Figure 3 shows that the mutant phenotype was unable to hydrolyze phosphate off phosphatidylinositol 4,5-bisphosphate |
complete | |||
See also
References
See Help:References for how to manage references in GONUTS.
