PMID:20607520

Citation	Balhesteros, H, Mazzon, RR, da Silva, CA, Lang, EA and Marques, MV (2010) CspC and CspD are essential for Caulobacter crescentus stationary phase survival. Arch. Microbiol. 192:747-58
Abstract	The cold shock response in bacteria involves the expression of low-molecular weight cold shock proteins (CSPs) containing a nucleic acid-binding cold shock domain (CSD), which are known to destabilize secondary structures on mRNAs, facilitating translation at low temperatures. Caulobacter crescentus cspA and cspB are induced upon cold shock, while cspC and cspD are induced during stationary phase. In this work, we determined a new coding sequence for the cspC gene, revealing that it encodes a protein containing two CSDs. The phenotypes of C. crescentus csp mutants were analyzed, and we found that cspC is important for cells to maintain viability during extended periods in stationary phase. Also, cspC and cspCD strains presented altered morphology, with frequent non-viable filamentous cells, and cspCD also showed a pronounced cell death at late stationary phase. In contrast, the cspAB mutant presented increased viability in this phase, which is accompanied by an altered expression of both cspC and cspD, but the triple cspABD mutant loses this characteristic. Taken together, our results suggest that there is a hierarchy of importance among the csp genes regarding stationary phase viability, which is probably achieved by a fine tune balance of the levels of these proteins.
Links	PubMed Online version:10.1007/s00203-010-0602-8
Keywords	Adaptation, Physiological; Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Caulobacter crescentus/genetics; Caulobacter crescentus/growth & development; Caulobacter crescentus/metabolism; Cold Temperature; DNA/genetics; Gene Deletion; Genes, Bacterial; Genetic Complementation Test; Heat-Shock Proteins/genetics; Heat-Shock Proteins/metabolism; Microbial Viability; Molecular Sequence Data; Sequence Analysis, DNA
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CAUCN:D9HP82		GO:0060274: maintenance of stationary phase	ECO:0000315:		P		3A	complete CACAO 11311
CAUCN:B8H564		GO:0070417: cellular response to cold	ECO:0000315:		P		Figure 3	complete
CAUCN:A0A0H3C7D5		GO:0060274: maintenance of stationary phase	ECO:0000316:	UniProtKB:D9HP82	P		Figure 3C shows that there is decreased viability of the cells.	complete CACAO 11325

References

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