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PMID:20545844
| Citation |
Catalão, MJ, Gil, F, Moniz-Pereira, J and Pimentel, M (2010) The mycobacteriophage Ms6 encodes a chaperone-like protein involved in the endolysin delivery to the peptidoglycan. Mol. Microbiol. 77:672-86 |
|---|---|
| Abstract |
Like most double-stranded (ds) DNA phages, mycobacteriophage Ms6 uses the holin-endolysin system to achieve lysis of its host. In addition to endolysin (lysA) and holin (hol) genes, Ms6 encodes three accessory lysis proteins. In this study we investigated the lysis function of Gp1, which is encoded by the gp1 gene that lies immediately upstream of lysA. Escherichia coli lysis was observed after coexpression of LysA and Gp1 in the absence of Ms6 holin. Gp1 does not belong to the holin class of proteins, and we provide evidence that it shares several characteristics with molecular chaperones. We show that Gp1 interacts with LysA, and that this interaction is necessary for LysA delivery to its target. In addition, PhoA fusions showed that, in Mycobacterium smegmatis, LysA is exported to the extracytoplasmic environment in the presence of Gp1. We also show that Gp1 is necessary for efficient M. smegmatis lysis, as Ms6 gp1 deletion results in host lysis defects. We propose that delivery of Ms6 endolysin to the murein layer is assisted by Gp1, a chaperone-like protein, in a holin-independent manner. |
| Links |
PubMed Online version:10.1111/j.1365-2958.2010.07239.x |
| Keywords |
Endopeptidases/genetics; Endopeptidases/metabolism; Escherichia coli/metabolism; Escherichia coli/virology; Molecular Chaperones/genetics; Molecular Chaperones/metabolism; Mycobacteriophages/genetics; Mycobacteriophages/metabolism; Mycobacterium smegmatis/metabolism; Mycobacterium smegmatis/virology; Peptidoglycan/metabolism; Protein Transport; Viral Proteins/genetics; Viral Proteins/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005579: membrane attack complex |
ECO:0000314: |
C |
gp1 of phage Ms6 is essential for efficient cell lysis. Figure 2 shows the activity of gp1 and the gene product LysA. In this figure you can clearly see that LysA is hardly performed or not able to perform at all when gp1 is absent. gp1 is NOT holin. Gp1 is the first thing inserted into the host cell by the phage, this opens a pore for ion and water flow that leads to cell lysis. |
complete | ||||
Notes
See also
References
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