PMID:20502699

Citation	Dieterle, MG, Wiest, AE, Plamann, M and McCluskey, K (2010) Characterization of the temperature-sensitive mutations un-7 and png-1 in Neurospora crassa. PLoS ONE 5:e10703
Abstract	The model filamentous fungus Neurospora crassa has been studied for over fifty years and many temperature-sensitive mutants have been generated. While most of these have been mapped genetically, many remain anonymous. The mutation in the N. crassa temperature-sensitive lethal mutant un-7 was identified by a complementation based approach as being in the open reading frame designated NCU00651 on linkage group I. Other mutations in this gene have been identified that lead to a temperature-sensitive morphological phenotype called png-1. The mutations underlying un-7 result in a serine to phenylalanine change at position 273 and an isoleucine to valine change at position 390, while the mutation in png-1 was found to result in a serine to leucine change at position 279 although there were other conservative changes in this allele. The overall morphology of the strain carrying the un-7 mutation is compared to strains carrying the png-1 mutation and these mutations are evaluated in the context of other temperature-sensitive mutants in Neurospora.
Links	PubMed PMC2872674 Online version:10.1371/journal.pone.0010703
Keywords	Amino Acid Sequence; Fungal Proteins/chemistry; Fungal Proteins/genetics; Genes, Fungal/genetics; Genetic Complementation Test; Molecular Sequence Data; Mutation/genetics; Neurospora crassa/genetics; Neurospora crassa/growth & development; Sequence Alignment; Temperature
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
NEUCR:PNG1		GO:0003684: damaged DNA binding	ECO:0000314:		F		The Figures in this paper provide the necessary evidence.	complete CACAO 10615

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