GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:20471426
| Citation |
Finzi, A, Pacheco, B, Zeng, X, Kwon, YD, Kwong, PD and Sodroski, J (2010) Conformational characterization of aberrant disulfide-linked HIV-1 gp120 dimers secreted from overexpressing cells. J. Virol. Methods 168:155-61 |
|---|---|
| Abstract |
The envelope (Env) glycoproteins of human immunodeficiency virus (HIV-1) mediate viral entry and are also the primary target of neutralizing antibodies. The gp160 envelope glycoprotein precursor undergoes proteolytic cleavage in the Golgi complex to produce the gp120 exterior glycoprotein and the gp41 transmembrane glycoprotein, which remain associated non-covalently in the trimeric Env complex. Monomeric soluble gp120 has been used extensively to investigate conformational states, structure, antigenicity and immunogenicity of the HIV-1 Env glycoproteins. Expression of gp120 alone (without gp41) leads to the accumulation not only of monomeric gp120 but also an aberrant dimeric form. The gp120 dimers were sensitive to reducing agents. The formation of gp120 dimers was disrupted by a single amino acid change in the inner domain, and was reduced by removal of the V1/V2 variable loops or the N and C termini. Epitopes on the gp120 inner domain and the chemokine receptor-binding surface were altered or occluded by gp120 dimerization. Awareness of the existence and properties of gp120 dimers should assist interpretation of studies of this key viral protein. |
| Links |
PubMed PMC2910153 Online version:10.1016/j.jviromet.2010.05.008 |
| Keywords |
Cell Line; Disulfides; HIV Envelope Protein gp120/chemistry; HIV-1/chemistry; Humans; Mutagenesis, Site-Directed; Protein Conformation; Protein Multimerization |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0019031: viral envelope |
ECO:0000315: |
C |
Fig. 3. Recognition of gp120 variants by CD4 and monoclonal antibodies. Note: gp160 cleaves into gp120 which is a surface protein and into gp41 which is a transmembrane protein. |
complete | ||||
See also
References
See Help:References for how to manage references in GONUTS.
