PMID:20400529

Citation	Yang, L, Ji, W, Zhu, Y, Gao, P, Li, Y, Cai, H, Bai, X and Guo, D (2010) GsCBRLK, a calcium/calmodulin-binding receptor-like kinase, is a positive regulator of plant tolerance to salt and ABA stress. J. Exp. Bot. 61:2519-33
Abstract	Calcium/calmodulin-dependent kinases play vital roles in protein phosphorylation in eukaryotes, yet little is known about the phosphorylation process of calcium/calmodulin-dependent protein kinase and its role in stress signal transduction in plants. A novel plant-specific calcium-dependent calmodulin-binding receptor-like kinase (GsCBRLK) has been isolated from Glycine soja. A subcellular localization study using GFP fusion protein indicated that GsCBRLK is localized in the plasma membrane. Binding assays demonstrated that calmodulin binds to GsCBRLK with an affinity of 25.9 nM in a calcium-dependent manner and the binding motif lies between amino acids 147 to169 within subdomain II of the kinase domain. GsCBRLK undergoes autophosphorylation and Myelin Basis Protein phosphorylation in the presence of calcium. It was also found that calcium/calmodulin positively regulates GsCBRLK kinase activity through direct interaction between the calmodulin-binding domain and calmodulin. So, it is likely that GsCBRLK responds to an environmental stimulus in two ways: by increasing the protein expression level and by regulating its kinase activity through the calcium/calmodulin complex. Furthermore, cold, salinity, drought, and ABA stress induce GsCBRLK gene transcripts. Over-expression of GsCBRLK in transgenic Arabidopsis resulted in enhanced plant tolerance to high salinity and ABA and increased the expression pattern of a number of stress gene markers in response to ABA and high salt. These results identify GsCBRLK as a molecular link between the stress- and ABA-induced calcium/calmodulin signal and gene expression in plant cells.
Links	PubMed Online version:10.1093/jxb/erq084
Keywords	Abscisic Acid/pharmacology; Amino Acid Sequence; Calcium/metabolism; Calcium-Calmodulin-Dependent Protein Kinases/chemistry; Calcium-Calmodulin-Dependent Protein Kinases/genetics; Calcium-Calmodulin-Dependent Protein Kinases/metabolism; Calmodulin/metabolism; Enzyme Stability; Gene Expression Regulation, Plant; Kinetics; Molecular Sequence Data; Protein Transport; Sequence Alignment; Sodium Chloride/metabolism; Soybean Proteins/chemistry; Soybean Proteins/genetics; Soybean Proteins/metabolism; Soybeans/drug effects; Soybeans/enzymology; Soybeans/genetics; Soybeans/physiology; Stress, Physiological
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
GLYSO:D8V833	part_of	GO:0005886: plasma membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
GLYSO:D8V833		GO:0005886: plasma membrane	ECO:0000314:		C		Figure 4	complete CACAO 8304

References

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