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PMID:20308291
| Citation |
Bouzahzah, B, Nagajyothi, F, Ghosh, K, Takvorian, PM, Cali, A, Tanowitz, HB and Weiss, LM (2010) Interactions of Encephalitozoon cuniculi polar tube proteins. Infect. Immun. 78:2745-53 |
|---|---|
| Abstract |
Microsporidia are eukaryotic, obligate intracellular organisms defined by small spores that contain a single invasion organelle, the polar tube, which coils around the interior of the spore. When these parasites infect host cells, the polar tube is discharged from the anterior pole of the spore, pierces the cell, and transfers sporoplasm into the cytoplasm of the host. Three polar tube proteins (PTP1, PTP2, and PTP3) have been identified in this structure. The interactions of these proteins in the assembly and function of the polar tube are not known. This study was undertaken to examine the protein interactions of the Encephalitozoon cuniculi polar tube proteins (EcPTPs). Immunofluorescence and immunoelectron microscopy confirmed the colocalization of EcPTP1, EcPTP2, and EcPTP3 to the polar tube. Experiments using cross-linkers indicated that EcPTP1, EcPTP2, and EcPTP3 form a complex in the polar tube, which was confirmed by immunoprecipitation using EcPTP1 antiserum. Yeast two-hybrid analysis revealed that full-length EcPTP1, EcPTP2, and EcPTP3 interact with each other in vivo. Both the N and C termini of EcPTP1 were involved in these interactions, but the central region of this protein, which contains a repetitive motif, was not. Further studies of polar tube proteins and their structural interactions may help elucidate the formation of the polar tube during the invasion process. |
| Links |
PubMed PMC2876541 Online version:10.1128/IAI.01205-09 |
| Keywords |
Encephalitozoon cuniculi/physiology; Fungal Proteins/metabolism; Immunoprecipitation; Microscopy, Fluorescence; Microscopy, Immunoelectron; Organelles/chemistry; Protein Binding; Protein Interaction Mapping; Two-Hybrid System Techniques |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0044099: polar tube |
ECO:0000314: |
C |
Fig 2a: polar tube incubated with anti-rEcPTP1 (binds to PTP1) fluoresces - indicates PTP1 is found on polar tube |
complete | ||||
|
part_of |
GO:0044099: polar tube |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0044099: polar tube |
ECO:0000314: |
C |
Fig 2d: polar tube incubated with anti-rEcPTP2 (binds to PTP2) fluoresces - indicates PTP2 is found on polar tube |
complete | ||||
|
part_of |
GO:0044099: polar tube |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0044099: polar tube |
ECO:0000314: |
C |
Fig 2b: polar tube incubated with anti-rEcPTP3 (binds to PTP3) fluoresces - indicates PTP3 is found on polar tube |
complete | ||||
|
part_of |
GO:0044099: polar tube |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
Notes
See also
References
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