PMID:20227482

Citation	Bearson, BL, Bearson, SM, Lee, IS and Brunelle, BW (2010) The Salmonella enterica serovar Typhimurium QseB response regulator negatively regulates bacterial motility and swine colonization in the absence of the QseC sensor kinase. Microb. Pathog. 48:214-9
Abstract	Salmonella enterica serovar Typhimurium (S. Typhimurium) responds to the catecholamine, norepinephrine by increasing bacterial growth and enhancing motility. In this study, iron with or without the siderophore, ferrioxamine E also enhanced bacterial motility. Iron-enhanced motility was growth-rate dependent, while norepinephrine-enhanced motility was growth-rate independent. The outer membrane catecholate receptors, IroN, FepA and CirA (required for norepinephrine-enhanced growth) were not required for norepinephrine-enhanced motility, nor was ExbD of the energy-transducing TonB-ExbB-ExbD ferri-siderophore uptake system. Examination of the QseBC two-component system revealed that qseB and qseBC mutants have motility phenotypes similar to wild-type S. Typhimurium, while motility of the qseC mutant was significantly decreased (P<0.01). Each mutant of the QseBC system, as well as mutants of qseE and pmrA, responded to norepinephrine with increased motility, suggesting that other genes are involved in norepinephrine-enhanced motility of S. Typhimurium. In the swine host, fecal shedding of the qseBC mutant was similar to wild-type S. Typhimurium, whereas fecal shedding of the qseC mutant was significantly decreased (P<0.01). Our data indicate that, in a qseC mutant, the QseB response regulator decreases motility and swine colonization; inactivation of the qseBC operon restores these bacterial phenotypes, classifying QseB as a negative regulator of bacterial motility and swine colonization.
Links	PubMed Online version:10.1016/j.micpath.2010.03.005
Keywords	Animals; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacterial Shedding; Chlorides/pharmacology; Feces/microbiology; Female; Ferric Compounds/pharmacology; Male; Mutation; Norepinephrine/pharmacology; Operon; Peptides, Cyclic/pharmacology; Quorum Sensing; Salmonella Infections/microbiology; Salmonella typhimurium/genetics; Salmonella typhimurium/physiology; Swine
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
SALTY:E7UUT8		GO:2000154: negative regulation of flagellar cell motility	IMP: Inferred from Mutant Phenotype:	P	Figure 2 shows relative motility levels of the WT and a QseC mutant.	complete CACAO 4938

References

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