PMID:20148901

Citation	Songsungthong, W, Higgins, MC, Rolán, HG, Murphy, JL and Mecsas, J (2010) ROS-inhibitory activity of YopE is required for full virulence of Yersinia in mice. Cell. Microbiol. 12:988-1001
Abstract	YopE, a type III secreted effector of Yersinia, is a GTPase Activating Protein for Rac1 and RhoA whose catalytic activity is critical for virulence. We found that YopE also inhibited reactive oxygen species (ROS) production and inactivated Rac2. How YopE distinguishes among its targets and which specific targets are critical for Yersinia survival in different tissues are unknown. A screen identifying YopE mutants in Yersinia pseudotuberculosis that interact with different Rho GTPases showed that YopE residues at positions 102, 106, 109 and 156 discern among switch I and II regions of Rac1, Rac2 and RhoA. Two mutants, which expressed YopE alleles with different antiphagocytic, ROS-inhibitory and cell-rounding activities, YptbL109A and YptbESptP, were studied in animal infections. Inhibition of both phagocytosis and ROS production were required for splenic colonization, whereas fewer YopE activities were required for Peyer's patch colonization. This study shows that Y. pseudotuberculosis encounters multiple host defences in different tissues and uses distinct YopE activities to disable them.
Links	PubMed PMC2897941 Online version:10.1111/j.1462-5822.2010.01448.x
Keywords	Animals; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/metabolism; Cell Line; Female; Humans; Mice; Mice, Inbred BALB C; Protein Binding; Reactive Oxygen Species/metabolism; Yersinia pseudotuberculosis/metabolism; Yersinia pseudotuberculosis/pathogenicity; rho GTP-Binding Proteins/metabolism
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
YERPE:YOPE	involved_in	GO:0050765: negative regulation of phagocytosis	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
YERPE:YOPE		GO:0050765: negative regulation of phagocytosis	ECO:0000315:		P		In table 1, shows mutant phenotypes with decreased negative regulation of phagocytosis.	complete CACAO 3307

References

See Help:References for how to manage references in GONUTS.