PMID:20035717

Citation	Lipson, RS, Webb, KJ and Clarke, SG (2010) Rmt1 catalyzes zinc-finger independent arginine methylation of ribosomal protein Rps2 in Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 391:1658-62
Abstract	Rps2/rpS2 is a well conserved protein of the eukaryotic ribosomal small subunit. Rps2 has previously been shown to contain asymmetric dimethylarginine residues, the addition of which is catalyzed by zinc-finger-containing arginine methyltransferase 3 (Rmt3) in the fission yeast Schizosaccharomyces pombe and protein arginine methyltransferase 3 (PRMT3) in mammalian cells. Here, we demonstrate that despite the lack of a zinc-finger-containing homolog of Rmt3/PRMT3 in the budding yeast Saccharomyces cerevisiae, Rps2 is partially modified to generate asymmetric dimethylarginine and monomethylarginine residues. We find that this modification of Rps2 is dependent upon the major arginine methyltransferase 1 (Rmt1) in S. cerevisiae. These results are suggestive of a role for Rmt1 in modifying the function of Rps2 in a manner distinct from that occurring in S. pombe and mammalian cells.
Links	PubMed PMC2813213 Online version:10.1016/j.bbrc.2009.12.112
Keywords	Amino Acid Sequence; Arginine/analogs & derivatives; Arginine/metabolism; Catalysis; Gene Deletion; Methylation; Molecular Sequence Data; Protein-Arginine N-Methyltransferases/genetics; Protein-Arginine N-Methyltransferases/metabolism; Repressor Proteins/genetics; Repressor Proteins/metabolism; Ribosomal Proteins/genetics; Ribosomal Proteins/metabolism; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Zinc Fingers
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