GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:20023035
| Citation |
Takacs, CN, Poggio, S, Charbon, G, Pucheault, M, Vollmer, W and Jacobs-Wagner, C (2010) MreB drives de novo rod morphogenesis in Caulobacter crescentus via remodeling of the cell wall. J. Bacteriol. 192:1671-84 |
|---|---|
| Abstract |
MreB, the bacterial actin-like cytoskeleton, is required for the rod morphology of many bacterial species. Disruption of MreB function results in loss of rod morphology and cell rounding. Here, we show that the widely used MreB inhibitor A22 causes MreB-independent growth inhibition that varies with the drug concentration, culture medium conditions, and bacterial species tested. MP265, an A22 structural analog, is less toxic than A22 for growth yet equally efficient for disrupting the MreB cytoskeleton. The action of A22 and MP265 is enhanced by basic pH of the culture medium. Using this knowledge and the rapid reversibility of drug action, we examined the restoration of rod shape in lemon-shaped Caulobacter crescentus cells pretreated with MP265 or A22 under nontoxic conditions. We found that reversible restoration of MreB function after drug removal causes extensive morphological changes including a remarkable cell thinning accompanied with elongation, cell branching, and shedding of outer membrane vesicles. We also thoroughly characterized the composition of C. crescentus peptidoglycan by high-performance liquid chromatography and mass spectrometry and showed that MreB disruption and recovery of rod shape following restoration of MreB function are accompanied by considerable changes in composition. Our results provide insight into MreB function in peptidoglycan remodeling and rod shape morphogenesis and suggest that MreB promotes the transglycosylase activity of penicillin-binding proteins. |
| Links |
PubMed PMC2832515 Online version:10.1128/JB.01311-09 |
| Keywords |
Bacterial Proteins/antagonists & inhibitors; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Caulobacter crescentus/cytology; Caulobacter crescentus/physiology; Cell Wall/ultrastructure; Cytoskeleton/metabolism; Hydrogen-Ion Concentration; Thiourea/analogs & derivatives; Thiourea/pharmacology |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0000902: cell morphogenesis |
ECO:0000315: |
P |
FIG. 3. Disruptive effects of A22 and MP265 treatments on GFP-MreB localization and rod morphology of C. crescentus., FIG. 4. Phenotypes associated with recovery from MP265 and A22 treatment., FIG. 5. Transmission electron micrographs of C. crescentus sacculi isolated at different time points during recovery from A22. |
complete | ||||
See also
References
See Help:References for how to manage references in GONUTS.
