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PMID:19930647
| Citation |
Stein, DC, Carrizosa, E and Dunham, S (2009) Use of nfsB, encoding nitroreductase, as a reporter gene to determine the mutational spectrum of spontaneous mutations in Neisseria gonorrhoeae. BMC Microbiol. 9:239 |
|---|---|
| Abstract |
Organisms that are sensitive to nitrofurantoin express a nitroreductase. Since bacterial resistance to this compound results primarily from mutations in the gene encoding nitroreductase, the resulting loss of function of nitroreductase results in a selectable phenotype; resistance to nitrofurantoin. We exploited this direct selection for mutation to study the frequency at which spontaneous mutations arise (transitions and transversions, insertions and deletions). |
| Links |
PubMed PMC2788575 Online version:10.1186/1471-2180-9-239 |
| Keywords |
Amino Acid Sequence; Anti-Infective Agents, Urinary/pharmacology; Drug Resistance, Bacterial/genetics; Genes, Reporter/genetics; Molecular Sequence Data; Mutation/genetics; Neisseria gonorrhoeae/drug effects; Neisseria gonorrhoeae/enzymology; Neisseria gonorrhoeae/genetics; Nitrofurantoin/pharmacology; Nitroreductases/chemistry; Nitroreductases/genetics; Nitroreductases/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
Contributes to |
GO:0018545: NAD(P)H nitroreductase activity |
ECO:0000315: |
F |
Figure 3 shows a reduction in nitroreductase activity in mutant phenotypes linked to alterations in nsfB |
complete | |||
See also
References
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