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PMID:19930647

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Citation

Stein, DC, Carrizosa, E and Dunham, S (2009) Use of nfsB, encoding nitroreductase, as a reporter gene to determine the mutational spectrum of spontaneous mutations in Neisseria gonorrhoeae. BMC Microbiol. 9:239

Abstract

Organisms that are sensitive to nitrofurantoin express a nitroreductase. Since bacterial resistance to this compound results primarily from mutations in the gene encoding nitroreductase, the resulting loss of function of nitroreductase results in a selectable phenotype; resistance to nitrofurantoin. We exploited this direct selection for mutation to study the frequency at which spontaneous mutations arise (transitions and transversions, insertions and deletions).

Links

PubMed PMC2788575 Online version:10.1186/1471-2180-9-239

Keywords

Amino Acid Sequence; Anti-Infective Agents, Urinary/pharmacology; Drug Resistance, Bacterial/genetics; Genes, Reporter/genetics; Molecular Sequence Data; Mutation/genetics; Neisseria gonorrhoeae/drug effects; Neisseria gonorrhoeae/enzymology; Neisseria gonorrhoeae/genetics; Nitrofurantoin/pharmacology; Nitroreductases/chemistry; Nitroreductases/genetics; Nitroreductases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CORDI:ODHI

Contributes to

GO:0018545: NAD(P)H nitroreductase activity

ECO:0000315:

F

Figure 3 shows a reduction in nitroreductase activity in mutant phenotypes linked to alterations in nsfB

complete
CACAO 4652


See also

References

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