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PMID:19906855
| Citation |
Hargitai, B, Kutnyánszky, V, Blauwkamp, TA, Steták, A, Csankovszki, G, Takács-Vellai, K and Vellai, T (2009) xol-1, the master sex-switch gene in C. elegans, is a transcriptional target of the terminal sex-determining factor TRA-1. Development 136:3881-7 |
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| Abstract |
In the nematode Caenorhabditis elegans, sex is determined by the ratio of X chromosomes to sets of autosomes: XX animals (2X:2A=1.0) develop as hermaphrodites and XO animals (1X:2A=0.5) develop as males. TRA-1, the worm ortholog of Drosophila Cubitus interruptus and mammalian Gli (Glioma-associated homolog) proteins, is the terminal transcription factor of the C. elegans sex-determination pathway, which specifies hermaphrodite fate by repressing male-specific genes. Here we identify a consensus TRA-1 binding site in the regulatory region of xol-1, the master switch gene controlling sex determination and dosage compensation. xol-1 is normally expressed in males, where it promotes male development and prevents dosage compensation. We show that TRA-1 binds to the consensus site in the xol-1 promoter in vitro and inhibits the expression of xol-1 in XX animals in vivo. Furthermore, inactivation of tra-1 enhances, whereas hyperactivation of tra-1 suppresses, lethality in animals with elevated xol-1 activity. These data imply the existence of a regulatory feedback loop within the C. elegans sex-determination and dosage-compensation cascade that ensures the accurate dose of X-linked genes in cells destined to adopt hermaphrodite fate. |
| Links |
PubMed PMC2778738 Online version:10.1242/dev.034637 |
| Keywords |
Animals; Base Sequence; Binding Sites; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Consensus Sequence; Conserved Sequence; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Dosage Compensation, Genetic; Genes, Helminth; Genes, Switch; Genes, X-Linked; Male; Molecular Sequence Data; Mutation; Promoter Regions, Genetic; Protein Binding; Sex Determination Processes; Transcription Factors/genetics; Transcription Factors/metabolism; X Chromosome |
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