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PMID:19816762
| Citation |
Li, G, Sun, C, Zhang, J, He, Y, Chen, H, Kong, J, Huang, G, Chen, K and Yao, Q (2009) Characterization of Bombyx mori parvo-like virus non-structural protein NS1. Virus Genes 39:396-402 |
|---|---|
| Abstract |
NS1 gene of Bombyx mori parvo-like virus (China Zhenjiang isolate, BmDNV-Z) codes a predicted 316-amino acid protein, but its function remains unknown. Results of the current study showed that purified recombinant 6 x His-NS1 protein possesses ATP binding, ATPase, DNA binding, and helicase activities. Only one protein was captured in infected Bombyx mori midgut cells against NS1 target protein by employing co-immunoprecipitation, which was identified to be a viral protein by mass spectrometry. The NS1-interacting protein is encoded by BmDNV-Z ORF4 and its molecular is about 100 kD. Analysis of His pull-down confirmed that binding of identified viral protein to purified recombinant 6 x His-NS1 protein in vitro. Taken together, our results indicated that BmDNV-Z NS1 was a multifunctional protein, which may be involved with virus replication. |
| Links |
PubMed Online version:10.1007/s11262-009-0402-x |
| Keywords |
Adenosine Triphosphatases/metabolism; Adenosine Triphosphate/metabolism; Amino Acid Sequence; Animals; Bombyx/virology; China; DNA/metabolism; DNA-Binding Proteins/metabolism; Immunoprecipitation; Mass Spectrometry; Molecular Sequence Data; Parvovirus/genetics; Parvovirus/isolation & purification; Protein Binding; Protein Interaction Mapping; Viral Nonstructural Proteins/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0016887: ATPase activity |
ECO:0000314: |
F |
Figure 3 To measure the ATPase activity a colorimetric malachite green assay was performed. The assay showed inorganic phosphate being released in the presence of the protein, compared to the little activity measured in the control. |
complete | ||||
| GO:0003678: DNA helicase activity |
ECO:0000314: |
F |
Figure 4 An displacement assay was used to test DNA helicase activity. Figure b Lane 2 shows it can unwind P1-54 dsDNA when ATP is added. |
complete | ||||
| GO:0003678: DNA helicase activity |
ECO:0000314: |
F |
Figure 4 An displacement assay was used to test DNA helicase activity. Figure b Lane 2 shows it can unwind P1-54 dsDNA when ATP is added. |
complete | ||||
| GO:0016887: ATPase activity |
ECO:0000314: |
F |
Figure 3 To measure the ATPase activity a colorimetric malachite green assay was performed. The assay showed inorganic phosphate being released in the presence of the protein, compared to the little activity measured in the control. |
complete | ||||
|
enables |
GO:0016887: ATPase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003678: DNA helicase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
Notes
See also
References
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