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PMID:19761831
| Citation |
Jonsson, A, Nordlund, S and Teixeira, PF (2009) Reduced activity of glutamine synthetase in Rhodospirillum rubrum mutants lacking the adenylyltransferase GlnE. Res. Microbiol. 160:581-4 |
|---|---|
| Abstract |
In the nitrogen-fixing bacterium Rhodospirillum rubrum, the GlnE adenylyltransferase (encoded by glnE) catalyzes reversible adenylylation of glutamine synthetase, thereby regulating nitrogen assimilation. We have generated glnE mutant strains that are unable to adenylylate glutamine synthetase (GS). Surprisingly, the activity of GS was lower in the mutants than in the wild type, even when grown in nitrogen-fixing conditions. Our results support the proposal that R. rubrum can only cope with the absence of an adenylylation system in the presence of lowered GS expression or activity. In general terms, this report also provides further support for the central role of GS in bacterial metabolism. |
| Links |
PubMed Online version:10.1016/j.resmic.2009.09.003 |
| Keywords |
Gene Deletion; Glutamate-Ammonia Ligase/metabolism; Nitrogen Fixation; Nucleotidyltransferases/genetics; Rhodospirillum rubrum/enzymology; Rhodospirillum rubrum/genetics |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
Contributes to |
GO:0006542: glutamine biosynthetic process |
ECO:0000314: |
P |
Figure 1: Activities of Glutamine Synthetase inferred from Western Blot Analysis |
complete | |||
|
involved_in |
GO:0006542: glutamine biosynthetic process |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0006542: glutamine biosynthetic process |
ECO:0000315: |
P |
See Figure 1. Summation of three GS activity in three different cultures. |
complete | ||||
| GO:0008882: (glutamate-ammonia-ligase) adenylyltransferase activity |
ECO:0000304: |
F |
GlnE adenylyltransferase to glutamine synthetase activity demonstrated through site mutation of glnE. Res Microbiol. 2009 Oct;160(8):581-4 Figure 1 shows activity and Table 2 dilneates site-mutations. PMID:19761831[1] |
complete | ||||
See also
References
See Help:References for how to manage references in GONUTS.
- ↑ Jonsson, A et al. (2009) Reduced activity of glutamine synthetase in Rhodospirillum rubrum mutants lacking the adenylyltransferase GlnE. Res. Microbiol. 160 581-4 PubMed GONUTS page
