PMID:1967980

Citation	Okamoto, K, Okazawa, H, Okuda, A, Sakai, M, Muramatsu, M and Hamada, H (1990) A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells. Cell 60:461-72
Abstract	We have identified a novel octamer binding factor (Oct-3) in P19 embryonal carcinoma cells. Oct-3, which recognizes the typical octamer motif (ATTTGCAT) as well as the AT-rich sequence TTAAAATTCA, is present in P19 stem cells but disappears when the cells are induced to differentiate by retinoic acid (RA). Cloned cDNA corresponding to Oct-3 encodes a protein of 377 amino acids. Oct-3 has a conserved POU domain, but the remaining part is distinct from other POU domain-containing proteins such as Oct-1 and Oct-2. mRNA of 1.5 kb coding for Oct-3 is abundant in P19 stem cells but is dramatically repressed during RA-induced differentiation. Repression of the 1.5 kb mRNA is rapid and specific to RA. In mouse, oct-3 mRNA is undetectable in all the adult organs examined. The N-terminal proline-rich region of Oct-3, when fused to the DNA binding domain of c-Jun, functions as a transcriptional activating domain. We suggest that Oct-3 is a novel octamer binding transcription factor that is developmentally regulated during mouse embryogenesis.
Links	PubMed
Keywords	Amino Acid Sequence; Animals; Base Sequence; Cell Line; Cloning, Molecular; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Enhancer Elements, Genetic; Gene Library; Genes, Homeobox; Genetic Vectors; Molecular Sequence Data; Nucleic Acid Hybridization; Octamer Transcription Factor-3; Oligonucleotide Probes; Plasmids; Protein Biosynthesis; RNA, Neoplasm/genetics; Sequence Homology, Nucleic Acid; Teratoma; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription, Genetic
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