PMID:19558586

Citation	Karlsson, R, Karlsson, A, Bäckman, O, Johansson, BR and Hulth, S (2009) Identification of key proteins involved in the anammox reaction. FEMS Microbiol. Lett. 297:87-94
Abstract	Bacteria performing anaerobic ammonium oxidation (anammox) are key players in the global nitrogen cycle due to their inherent ability to convert biologically available nitrogen to N(2). Anammox is increasingly being exploited during wastewater treatment worldwide, and about 50% of the total N(2) production in marine environments is estimated to proceed by the anammox pathway. To fully understand the microbial functionality and mechanisms that control environmental feedbacks of the anammox reaction, key proteins involved in the reaction must be identified. In this study we have utilized an analytical protocol that facilitates detection of proteins associated with the anammoxosome, an intracellular membrane compartment within the anammox bacterium. The protocol enabled us to identify several key proteins of the anammox reaction including a hydrazine hydrolase producing hydrazine, a hydrazine-oxidizing enzyme converting hydrazine to N(2) and a membrane-bound ATP synthase generating ATP from the gradients of protons formed in the anammox reaction. We also performed immunogold labelling electron microscopy to determine the subcellular location of the hydrazine hydrolase. The results from our study support the hypothesis that proteins associated with the anammoxosome host the complete suite of reactions during anammox.
Links	PubMed Online version:10.1111/j.1574-6968.2009.01677.x
Keywords	Anaerobiosis; Bacteria/enzymology; Bacteria/genetics; Bacteria/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Hydrazines/metabolism; Nitrogen/metabolism; Oxidation-Reduction; Quaternary Ammonium Compounds/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9BACT:Q1Q0T3		GO:0044222: anammoxosome	ECO:0000314:		C		Results: "The anammoxosomes of intact bacteria were invariably labelled with the anti-kuste2860 and anti-kuste2861 antibodies, whereas no labelling was observed in the cytosol outside the membranous compartment (Fig. 3a). This confirmed a clear association of the hydrazine hydrolase with the anammoxosomes."	complete CACAO 2363
9BACT:Q1Q0T3	part_of	GO:0044222: anammoxosome	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
9BACT:Q1Q3H0	part_of	GO:0016469: proton-transporting two-sector ATPase complex	ECO:0000255: match to sequence model evidence used in manual assertion		C		Seeded From UniProt	Missing: with/from
9BACT:Q1Q3H0		GO:0016469: proton-transporting two-sector ATPase complex	ECO:0000255:	PMID:9254694	C		Table 1.	complete CACAO 2364
9BACT:Q1Q3H2		GO:0016469: proton-transporting two-sector ATPase complex	ECO:0000255:	PMID:9254694	C		Table 1.	complete CACAO 2365
9BACT:Q1Q3H2	part_of	GO:0016469: proton-transporting two-sector ATPase complex	ECO:0000255: match to sequence model evidence used in manual assertion		C		Seeded From UniProt	Missing: with/from

References

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