PMID:19457051

Citation	Wu, Z, Li, Y, Pan, G, Zhou, Z and Xiang, Z SWP25, a novel protein associated with the Nosema bombycis endospore. J. Eukaryot. Microbiol. 56:113-8
Abstract	Microsporidia are eukaryotic, obligate intracellular, spore-forming parasites. The resistant spores, which harbor a rigid cell wall, are critical for their host-to-host transmission and persistence in the environment. The spore wall comprises two major layers: the exospore and the endospore. In Nosema bombycis, two spore wall proteins have been characterized--an endosporal protein, SWP30, and an exosporal protein, SWP32. Here, we report the identification of the third spore wall protein of N. bombycis, SWP25, the gene of which has no known homologue. SWP25 is predicted to posses a signal peptide and a heparin-binding motif. Immunoelectron microscopy analysis showed that this protein is localized to the endospore. This characterization of a new spore wall protein of N. bombycis may facilitate our investigation of the relationship between N. bombycis and its host, Bombyx mori.
Links	PubMed Online version:10.1111/j.1550-7408.2008.00375.x
Keywords	Amino Acid Sequence; Animals; Antibodies, Fungal; Bombyx/microbiology; Cell Wall/chemistry; Fluorescent Antibody Technique; Fungal Proteins/chemistry; Fungal Proteins/isolation & purification; Fungal Proteins/metabolism; Host-Pathogen Interactions; Membrane Proteins/isolation & purification; Molecular Sequence Data; Nosema/chemistry; Nosema/cytology; Nosema/metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spores, Fungal/chemistry
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
NOSB1:SWP25		GO:0043593: endospore coat	ECO:0000314:		C		Figure 5-8 show immunogold imaging of SWP25 localized to the endospore.	complete CACAO 10936

References

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