GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com


Jump to: navigation, search

Rumbak, E, Rawlings, DE, Lindsey, GG and Woods, DR (1991) Characterization of the Butyrivibrio fibrisolvens glgB gene, which encodes a glycogen-branching enzyme with starch-clearing activity. J. Bacteriol. 173:6732-41


A Butyrivibrio fibrisolvens H17c glgB gene, was isolated by direct selection for colonies that produced clearing on starch azure plates. The gene was expressed in Escherichia coli from its own promoter. The glgB gene consisted of an open reading frame of 1,920 bp encoding a protein of 639 amino acids (calculated Mr, 73,875) with 46 to 50% sequence homology with other branching enzymes. A limited region of 12 amino acids showed sequence similarity to amylases and glucanotransferases. The B. fibrisolvens branching enzyme was not able to hydrolyze starch but stimulated phosphorylase alpha-mediated incorporation of glucose into alpha-1,4-glucan polymer 13.4-fold. The branching enzyme was purified to homogeneity by a simple two-step procedure; N-terminal sequence and amino acid composition determinations confirmed the deduced translational start and amino acid sequence of the open reading frame. The enzymatic properties of the purified enzyme were investigated. The enzyme transferred chains of 5 to 10 (optimum, 7) glucose units, using amylose and amylopetin as substrates, to produce a highly branched polymer.


PubMed PMC209022


1,4-alpha-Glucan Branching Enzyme/genetics; 1,4-alpha-Glucan Branching Enzyme/isolation & purification; 1,4-alpha-Glucan Branching Enzyme/metabolism; Amino Acid Sequence; Animals; Bacteroidaceae/enzymology; Bacteroidaceae/genetics; Base Sequence; Carbohydrate Sequence; Chromatography, DEAE-Cellulose; DNA, Bacterial; Electrophoresis, Polyacrylamide Gel; Genes, Bacterial; Glucans/metabolism; Kinetics; Molecular Sequence Data; Restriction Mapping; Rumen/microbiology; Sequence Alignment; Starch/metabolism



Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status


GO:0003844: 1,4-alpha-glucan branching enzyme activity



Figure 9 shows the absorbance spectra of the iodine-glucan complex of the a-glucan formed de novo by the purified branching enzyme and phosphorylase a (ratio of 1:1). The combination of the actions of phosphorylase a and GlgB yielded a branched product with a spectrum similar to glycogen. The initial Xmax was 490nm, but as the reaction proceeded decreased to 430nm, indicating a highly branched product.

CACAO 4873



GO:0003844: 1,4-alpha-glucan branching enzyme activity

ECO:0000314: direct assay evidence used in manual assertion


Seeded From UniProt


See also


See Help:References for how to manage references in GONUTS.