PMID:19074312

Citation	Mihola, O, Trachtulec, Z, Vlcek, C, Schimenti, JC and Forejt, J (2009) A mouse speciation gene encodes a meiotic histone H3 methyltransferase. Science 323:373-5
Abstract	Speciation genes restrict gene flow between the incipient species and related taxa. Three decades ago, we mapped a mammalian speciation gene, hybrid sterility 1 (Hst1), in the intersubspecific hybrids of house mouse. Here, we identify this gene as Prdm9, encoding a histone H3 lysine 4 trimethyltransferase. We rescued infertility in male hybrids with bacterial artificial chromosomes carrying Prdm9 from a strain with the "fertility" Hst1(f) allele. Sterile hybrids display down-regulated microrchidia 2B (Morc2b) and fail to compartmentalize gammaH2AX into the pachynema sex (XY) body. These defects, seen also in Prdm9-null mutants, are rescued by the Prdm9 transgene. Identification of a vertebrate hybrid sterility gene reveals a role for epigenetics in speciation and opens a window to a hybrid sterility gene network.
Links	PubMed Online version:10.1126/science.1163601
Keywords	Amino Acid Sequence; Animals; Chromosome Mapping; Chromosomes, Artificial, Bacterial; Crosses, Genetic; Epigenesis, Genetic; Female; Gene Expression Regulation; Genetic Speciation; Histone-Lysine N-Methyltransferase/chemistry; Histone-Lysine N-Methyltransferase/genetics; Histone-Lysine N-Methyltransferase/metabolism; Histones/metabolism; Hybridization, Genetic; Infertility, Male/genetics; Male; Meiosis; Methylation; Mice; Mice, Inbred C3H; Mice, Transgenic; Molecular Sequence Data; Ovary/enzymology; Testis/enzymology
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Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
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