PMID:19054069

Citation	Yamada, A, Tamura, H and Kato, H (2009) Identification and characterization of an autolysin gene, atlg, from Streptococcus sobrinus. FEMS Microbiol. Lett. 291:17-23
Abstract	AtlA is a major cell-lytic enzyme called autolysin in Streptococcus mutans. In this study, we identified the atlg gene-encoding autolysin (Atlg), consisting of 863 residues from Streptococcus sobrinus 6715DP, and confirmed lytic activity of recombinant Atlg by zymography of S. sobrinus cells. An atlA-inactivated mutant was constructed in S. mutans Xc, and the atlg gene product was characterized by plasmid complementation. Microscopic analysis, saliva-induced aggregation assay and autolysis assay of static cultures in air revealed that the atlg gene product partially complemented the role of AtlA. Furthermore, the capability of biofilm formation of the atlA-deficient mutant cultivated in air was restored by plasmid comprising the atlg gene. These findings suggest that Atlg may be involved in cell separation and biofilm formation in S. sobrinus.
Links	PubMed Online version:10.1111/j.1574-6968.2008.01426.x
Keywords	Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biofilms; Humans; Molecular Sequence Data; N-Acetylmuramoyl-L-alanine Amidase/chemistry; N-Acetylmuramoyl-L-alanine Amidase/genetics; N-Acetylmuramoyl-L-alanine Amidase/metabolism; Saliva/microbiology; Sequence Alignment; Streptococcus mutans/genetics; Streptococcus mutans/metabolism; Streptococcus sobrinus/chemistry; Streptococcus sobrinus/enzymology; Streptococcus sobrinus/genetics; Streptococcus sobrinus/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9STRE:A7VLR4		GO:0001896: autolysis	ECO:0000315:		P		Fig. 5 shows that Atlg could act as an autolysin of S. mutans	complete CACAO 8757

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