PMID:19016776

Citation	Mahajan, A, Currie, CG, Mackie, S, Tree, J, McAteer, S, McKendrick, I, McNeilly, TN, Roe, A, La Ragione, RM, Woodward, MJ, Gally, DL and Smith, DG (2009) An investigation of the expression and adhesin function of H7 flagella in the interaction of Escherichia coli O157 : H7 with bovine intestinal epithelium. Cell. Microbiol. 11:121-37
Abstract	Enterohaemorrhagic Escherichia coli O157 : H7 is a bacterial pathogen that can cause haemorrhagic colitis and haemolytic uremic syndrome. In the primary reservoir host, cattle, the terminal rectum is the principal site of E. coli O157 colonization. In this study, bovine terminal rectal primary epithelial cells were used to examine the role of H7 flagella in epithelial adherence. Binding of a fliC(H7) mutant O157 strain to rectal epithelium was significantly reduced as was binding of the flagellated wild-type strain following incubation with H7-specific antibodies. Complementation of fliC(H7) mutant O157 strain with fliC(H7) restored the adherence to wild-type levels; however, complementation with fliC(H6) did not restore it. High-resolution ultrastructural and imunofluorescence studies demonstrated the presence of abundant flagella forming physical contact points with the rectal epithelium. Binding to terminal rectal epithelium was specific to H7 by comparison with other flagellin types tested. In-cell Western assays confirmed temporal expression of flagella during O157 interaction with epithelium, early expression was suppressed during the later stages of microcolony and attaching and effacing lesion formation. H7 flagella are expressed in vivo by individual bacteria in contact with rectal mucosa. Our data demonstrate that the H7 flagellum acts as an adhesin to bovine intestinal epithelium and its involvement in this crucial initiating step for colonization indicates that H7 flagella could be an important target in intervention strategies.
Links	PubMed Online version:10.1111/j.1462-5822.2008.01244.x
Keywords	Adhesins, Bacterial/metabolism; Animals; Bacterial Adhesion; Cattle; Cells, Cultured; Epithelial Cells/microbiology; Escherichia coli O157/physiology; Escherichia coli O157/ultrastructure; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Flagella/physiology; Flagella/ultrastructure; Gene Expression Profiling; Genetic Complementation Test; Intestinal Mucosa/microbiology; Mutation
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ECO57:Q7DBI0		GO:0007155: cell adhesion	ECO:0000315:		P		Figure 1 shows that the mutant strain without the fliC gene had significantly less adhesion ability than the wild-type strain.	complete CACAO 10080
ECO57:Q7DBI0	involved_in	GO:0007155: cell adhesion	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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