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PMID:18847457
Citation |
Portakal, O and Doğan, P (2008) Construction of recB-recD genetic fusion and functional analysis of RecBDC fusion enzyme in Escherichia coli. BMC Biochem. 9:27 |
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Abstract |
recD, located between recB and argA, encodes the smallest polypeptide (60 kDa) of the heterotrimeric enzyme RecBCD in Escherichia coli. RecD is a 5'-3' helicase and is required for the nuclease activity of RecBCD and for tight binding to dsDNA ends. Here, we have tested the hypothesis that RecD regulates the structure and activities of RecBCD, including RecA loading. |
Links |
PubMed PMC2586629 Online version:10.1186/1471-2091-9-27 |
Keywords |
Alleles; Base Sequence; DNA, Bacterial/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Exodeoxyribonuclease V/genetics; Exodeoxyribonuclease V/metabolism; Exodeoxyribonuclease V/physiology; Haploidy; Models, Biological; Molecular Sequence Data; Mutation; Phenotype; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Recombination, Genetic |
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Significance
Annotations
Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
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See also
References
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