PMID:18809219

Citation	Obeso, JM, Martínez, B, Rodríguez, A and García, P (2008) Lytic activity of the recombinant staphylococcal bacteriophage PhiH5 endolysin active against Staphylococcus aureus in milk. Int. J. Food Microbiol. 128:212-8
Abstract	The endolysin gene (lysH5) from the genome of the Staphylococcus aureus bacteriophage PhiH5 was cloned in Escherichia coli and characterized. The lysH5 gene encoded a protein (LysH5) whose calculated molecular mass and pI were 53.7 kDa and 8.7, respectively. Comparative analysis revealed that LysH5 significantly resembled other murein hydrolases encoded by staphylococcal phages. The modular organization of LysH5 comprised three putative domains, namely, CHAP (cysteine, histidine-dependent amidohydrolase/peptidase), amidase (L-muramoyl-L-alanine amidase), and SH3b (cell wall recognition). In turbidity reduction assays, the purified protein lysed bovine and human S. aureus, and human Staphylococcus epidermidis strains. Other bacteria belonging to different genera were not affected. The lytic activity was optimal at pH 7.0, 37 degrees C, and sensitive to high temperatures. The purified protein was able to kill rapidly S. aureus growing in pasteurized milk and the pathogen was not detected after 4 h of incubation at 37 degrees C. As far as we know, this is the first report to assess the antimicrobial activity of a phage endolysin which might be useful for novel biocontrol strategies in dairying.
Links	PubMed Online version:10.1016/j.ijfoodmicro.2008.08.010
Keywords	Amidohydrolases/genetics; Amidohydrolases/metabolism; Animals; Cattle; Cloning, Molecular; Endopeptidases/chemistry; Endopeptidases/genetics; Endopeptidases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/virology; Female; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Viral; Humans; Hydrogen-Ion Concentration; Membrane Proteins/chemistry; Membrane Proteins/genetics; Membrane Proteins/metabolism; Milk/metabolism; Milk/microbiology; Milk/virology; N-Acetylmuramoyl-L-alanine Amidase/genetics; N-Acetylmuramoyl-L-alanine Amidase/metabolism; Peptide Hydrolases/genetics; Peptide Hydrolases/metabolism; Recombinant Fusion Proteins/biosynthesis; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/metabolism; Staphylococcus Phages/enzymology; Staphylococcus Phages/genetics; Staphylococcus Phages/metabolism; Staphylococcus aureus/metabolism; Staphylococcus aureus/virology; Temperature; Viral Proteins/chemistry; Viral Proteins/genetics; Viral Proteins/metabolism
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9CAUD:B8QIR1		GO:0044659: cytolysis by virus of host cell	ECO:0000314:		P		Figure 3 shows lysis of Staphylococcus aureus cells by LysH5.	complete CACAO 10743
9CAUD:B8QIR1	involved_in	GO:0044659: cytolysis by virus of host cell	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.