PMID:18799621

Citation	Alvarez, FJ, Douglas, LM, Rosebrock, A and Konopka, JB (2008) The Sur7 protein regulates plasma membrane organization and prevents intracellular cell wall growth in Candida albicans. Mol. Biol. Cell 19:5214-25
Abstract	The Candida albicans plasma membrane plays important roles in cell growth and as a target for antifungal drugs. Analysis of Ca-Sur7 showed that this four transmembrane domain protein localized to stable punctate patches, similar to the plasma membrane subdomains known as eisosomes or MCC that were discovered in S. cerevisiae. The localization of Ca-Sur7 depended on sphingolipid synthesis. In contrast to S. cerevisiae, a C. albicans sur7Delta mutant displayed defects in endocytosis and morphogenesis. Septins and actin were mislocalized, and cell wall synthesis was very abnormal, including long projections of cell wall into the cytoplasm. Several phenotypes of the sur7Delta mutant are similar to the effects of inhibiting beta-glucan synthase, suggesting that the abnormal cell wall synthesis is related to activation of chitin synthase activity seen under stress conditions. These results expand the roles of eisosomes by demonstrating that Sur7 is needed for proper plasma membrane organization and cell wall synthesis. A conserved Cys motif in the first extracellular loop of fungal Sur7 proteins is similar to a characteristic motif of the claudin proteins that form tight junctions in animal cells, suggesting a common role for these tetraspanning membrane proteins in forming specialized plasma membrane domains.
Links	PubMed PMC2592640 Online version:10.1091/mbc.E08-05-0479
Keywords	Actins/metabolism; Amino Acid Sequence; Candida albicans/cytology; Candida albicans/metabolism; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cell Membrane/metabolism; Cell Membrane/ultrastructure; Cell Wall/physiology; Cell Wall/ultrastructure; Cytoskeletal Proteins/genetics; Cytoskeletal Proteins/metabolism; Endocytosis/physiology; Fungal Proteins/genetics; Fungal Proteins/metabolism; Gene Expression Profiling; Humans; Hyphae/physiology; Hyphae/ultrastructure; Membrane Proteins/genetics; Membrane Proteins/metabolism; Molecular Sequence Data; Morphogenesis; Oligonucleotide Array Sequence Analysis; Phosphoproteins/genetics; Phosphoproteins/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Sequence Alignment
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