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PMID:18694567
| Citation |
Soper, SF, van der Heijden, GW, Hardiman, TC, Goodheart, M, Martin, SL, de Boer, P and Bortvin, A (2008) Mouse maelstrom, a component of nuage, is essential for spermatogenesis and transposon repression in meiosis. Dev. Cell 15:285-97 |
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| Abstract |
Tight control of transposon activity is essential for the integrity of the germline. Recently, a germ-cell-specific organelle, nuage, was proposed to play a role in transposon repression. To test this hypothesis, we disrupted a murine homolog of a Drosophila nuage protein Maelstrom. Effects on male meiotic chromosome synapsis and derepression of transposable elements (TEs) were observed. In the adult Mael(-/-) testes, LINE-1 (L1) derepression occurred at the onset of meiosis. As a result, Mael(-/-) spermatocytes were flooded with L1 ribonucleoproteins (RNPs) that accumulated in large cytoplasmic enclaves and nuclei. Mael(-/-) spermatocytes with nuclear L1 RNPs exhibited massive DNA damage and severe chromosome asynapsis even in the absence of SPO11-generated meiotic double-strand breaks. This study demonstrates that MAEL, a nuage component, is indispensable for the silencing of TEs and identifies the initiation of meiosis as an important step in TE control in the male germline. |
| Links |
PubMed PMC2546488 Online version:10.1016/j.devcel.2008.05.015 |
| Keywords |
Alleles; Animals; Cell Nucleus Structures/metabolism; Cell Nucleus Structures/ultrastructure; Chromosome Pairing; Chromosomes, Mammalian/metabolism; DNA Breaks, Double-Stranded; DNA Methylation; DNA Transposable Elements; DNA-Binding Proteins/metabolism; Endodeoxyribonucleases; Esterases/metabolism; Gene Targeting; Male; Meiosis; Mice; Mice, Inbred C57BL; Open Reading Frames/genetics; Protein Transport; Ribonucleoproteins/metabolism; Sex Chromosomes/metabolism; Spermatocytes/cytology; Spermatocytes/ultrastructure; Spermatogenesis; Spermatozoa/cytology; Spermatozoa/ultrastructure; Testis/cytology; Testis/ultrastructure; Transcription Factors/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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