GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:18676877

From GONUTS
Jump to: navigation, search
Citation

Andème Ondzighi, C, Christopher, DA, Cho, EJ, Chang, SC and Staehelin, LA (2008) Arabidopsis protein disulfide isomerase-5 inhibits cysteine proteases during trafficking to vacuoles before programmed cell death of the endothelium in developing seeds. Plant Cell 20:2205-20

Abstract

Protein disulfide isomerase (PDI) oxidizes, reduces, and isomerizes disulfide bonds, modulates redox responses, and chaperones proteins. The Arabidopsis thaliana genome contains 12 PDI genes, but little is known about their subcellular locations and functions. We demonstrate that PDI5 is expressed in endothelial cells about to undergo programmed cell death (PCD) in developing seeds. PDI5 interacts with three different Cys proteases in yeast two-hybrid screens. One of these traffics together with PDI5 from the endoplasmic reticulum through the Golgi to vacuoles, and its recombinant form is functionally inhibited by recombinant PDI5 in vitro. Peak PDI5 expression in endothelial cells precedes PCD, whereas decreasing PDI5 levels coincide with the onset of PCD-related cellular changes, such as enlargement and subsequent collapse of protein storage vacuoles, lytic vacuole shrinkage and degradation, and nuclear condensation and fragmentation. Loss of PDI5 function leads to premature initiation of PCD during embryogenesis and to fewer, often nonviable, seeds. We propose that PDI5 is required for proper seed development and regulates the timing of PCD by chaperoning and inhibiting Cys proteases during their trafficking to vacuoles before PCD of the endothelial cells. During this transitional phase of endothelial cell development, the protein storage vacuoles become the de facto lytic vacuoles that mediate PCD.

Links

PubMed PMC2553623 Online version:10.1105/tpc.108.058339

Keywords

Amino Acid Sequence; Apoptosis/genetics; Apoptosis/physiology; Arabidopsis/cytology; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cysteine Endopeptidases/genetics; Cysteine Endopeptidases/metabolism; Electrophoresis, Polyacrylamide Gel; Immunoblotting; Microscopy, Electron, Transmission; Molecular Sequence Data; Plants, Genetically Modified/genetics; Plants, Genetically Modified/metabolism; Plants, Genetically Modified/ultrastructure; Protein Disulfide-Isomerases/genetics; Protein Disulfide-Isomerases/metabolism; Protein Transport; Reverse Transcriptase Polymerase Chain Reaction; Seeds/cytology; Seeds/genetics; Seeds/metabolism; Two-Hybrid System Techniques; Vacuoles/metabolism; Vacuoles/ultrastructure

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

See Help:References for how to manage references in GONUTS.