PMID:18656453

Citation	Minehata, K, Kawahara, A and Suzuki, T (2008) meis1 regulates the development of endothelial cells in zebrafish. Biochem. Biophys. Res. Commun. 374:647-52
Abstract	The differentiation of endothelial cells is tightly connected with the formation of blood vessels during vertebrate development. The signaling pathways mediated by vascular endothelial growth factor (vegf) are required for these processes. Here we show that a proto-oncogene, meis1, plays important roles in the vascular development in zebrafish. Knockdown of meis1 by anti-sense meis1 morpholino (meis1 MO) led to the impairment of intersegmental vessel (ISV) formation. In meis1 morphants, the expression of an artery marker was reduced in dorsal aorta (DA), and the expression of vein markers was expanded in DA and posterior cardinal vein (PCV), suggesting the defects on artery development. Furthermore, the expression of vegf receptor, flk1, was significantly decreased in these embryos. Interestingly, flk1 MO-injected embryos exhibited similar defects as meis1 morphants. Thus, these results implicate that meis1 is a novel regulator involved in endothelial cell development, presumably affecting the vegf signaling pathway.
Links	PubMed Online version:10.1016/j.bbrc.2008.07.075
Keywords	Animals; Blood Vessels/cytology; Blood Vessels/embryology; Blood Vessels/metabolism; Cell Differentiation; Embryonic Development; Endothelium, Vascular/cytology; Endothelium, Vascular/embryology; Endothelium, Vascular/metabolism; Phenotype; RNA, Antisense/genetics; Transcription Factors/genetics; Transcription Factors/physiology; Vascular Endothelial Growth Factor Receptor-2/genetics; Vascular Endothelial Growth Factor Receptor-2/metabolism; Zebrafish/embryology; Zebrafish/genetics; Zebrafish/immunology; Zebrafish Proteins/genetics; Zebrafish Proteins/physiology
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