PMID:18635795

Citation	Koh, K, Joiner, WJ, Wu, MN, Yue, Z, Smith, CJ and Sehgal, A (2008) Identification of SLEEPLESS, a sleep-promoting factor. Science 321:372-6
Abstract	Sleep is an essential process conserved from flies to humans. The importance of sleep is underscored by its tight homeostatic control. Through a forward genetic screen, we identified a gene, sleepless, required for sleep in Drosophila. The sleepless gene encodes a brain-enriched, glycosylphosphatidylinositol-anchored protein. Loss of SLEEPLESS protein caused an extreme (>80%) reduction in sleep; a moderate reduction in SLEEPLESS had minimal effects on baseline sleep but markedly reduced the amount of recovery sleep after sleep deprivation. Genetic and molecular analyses revealed that quiver, a mutation that impairs Shaker-dependent potassium current, is an allele of sleepless. Consistent with this finding, Shaker protein levels were reduced in sleepless mutants. We propose that SLEEPLESS is a signaling molecule that connects sleep drive to lowered membrane excitability.
Links	PubMed PMC2771549 Online version:10.1126/science.1155942
Keywords	Amino Acid Sequence; Animals; Animals, Genetically Modified; Behavior, Animal; Brain/metabolism; Cell Membrane/metabolism; DNA Transposable Elements; Drosophila Proteins/chemistry; Drosophila Proteins/genetics; Drosophila Proteins/physiology; Drosophila melanogaster/genetics; Drosophila melanogaster/physiology; Female; Genes, Insect; Glycosylphosphatidylinositols; Homeostasis; Longevity; Male; Membrane Proteins/chemistry; Membrane Proteins/genetics; Membrane Proteins/physiology; Models, Animal; Molecular Sequence Data; Mutation; Phenotype; Shaker Superfamily of Potassium Channels/physiology; Signal Transduction; Sleep/genetics; Sleep/physiology; Sleep Deprivation; Transgenes
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Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
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