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PMID:18633119
| Citation |
Rutschow, H, Ytterberg, AJ, Friso, G, Nilsson, R and van Wijk, KJ (2008) Quantitative proteomics of a chloroplast SRP54 sorting mutant and its genetic interactions with CLPC1 in Arabidopsis. Plant Physiol. 148:156-75 |
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| Abstract |
cpSRP54 (for chloroplast SIGNAL RECOGNITION PARTICLE54) is involved in cotranslational and posttranslational sorting of thylakoid proteins. The Arabidopsis (Arabidopsis thaliana) cpSRP54 null mutant, ffc1-2, is pale green with delayed development. Western-blot analysis of individual leaves showed that the SRP sorting pathway, but not the SecY/E translocon, was strongly down-regulated with progressive leaf development in both wild-type and ffc1-2 plants. To further understand the impact of cpSRP54 deletion, a quantitative comparison of ffc2-1 was carried out for total leaf proteomes of young seedlings and for chloroplast proteomes of fully developed leaves using stable isotope labeling (isobaric stable isotope labeling and isotope-coded affinity tags) and two-dimensional gels. This showed that cpSRP54 deletion led to a change in light-harvesting complex composition, an increase of PsbS, and a decreased photosystem I/II ratio. Moreover, the cpSRP54 deletion led in young leaves to up-regulation of thylakoid proteases and stromal chaperones, including ClpC. In contrast, the stromal protein homeostasis machinery returned to wild-type levels in mature leaves, consistent with the developmental down-regulation of the SRP pathway. A differential response between young and mature leaves was also found in carbon metabolism, with an up-regulation of the Calvin cycle and the photorespiratory pathway in peroxisomes and mitochondria in young leaves but not in old leaves. The Calvin cycle was down-regulated in mature leaves to adjust to the reduced capacity of the light reaction, while reactive oxygen species defense proteins were up-regulated. The significance of ClpC up-regulation was confirmed through the generation of an ffc2-1 clpc1 double mutant. This mutant was seedling lethal under autotrophic conditions but could be partially rescued under heterotrophic conditions. |
| Links |
PubMed PMC2528104 Online version:10.1104/pp.108.124545 |
| Keywords |
Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Chloroplast Proteins; Chloroplasts/metabolism; Cytosol/metabolism; Electrophoresis, Gel, Two-Dimensional; Endopeptidase Clp/genetics; Endopeptidase Clp/metabolism; Homeostasis; Isoelectric Focusing; Isotopes/metabolism; Light; Light-Harvesting Protein Complexes/metabolism; Mass Spectrometry; Mutation; Nitrogen/metabolism; Photosynthesis; Photosystem II Protein Complex/metabolism; Plant Leaves/metabolism; Plant Proteins/biosynthesis; Protein Transport; Proteomics; Seedling/growth & development; Seedling/metabolism; Signal Recognition Particle/genetics; Signal Recognition Particle/metabolism; Thylakoids/metabolism |
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Significance
Annotations
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