PMID:18566389

Citation	Bonfield, TL, Thomassen, MJ, Farver, CF, Abraham, S, Koloze, MT, Zhang, X, Mosser, DM and Culver, DA (2008) Peroxisome proliferator-activated receptor-gamma regulates the expression of alveolar macrophage macrophage colony-stimulating factor. J. Immunol. 181:235-42
Abstract	Macrophage CSF (M-CSF) regulates monocyte differentiation, activation, and foam cell formation. We have observed that it is elevated in human pulmonary alveolar proteinosis (PAP) and in the GM-CSF knockout mouse, a murine model for PAP. A potential regulator of M-CSF, peroxisome proliferator-activated receptor-gamma (PPARgamma), is severely deficient in both human PAP and the GM-CSF knockout mouse. To investigate the role of PPARgamma in alveolar macrophage homeostasis, we generated myeloid-specific PPARgamma knockout mice using the Lys-Cre method to knock out the floxed PPARgamma gene. Similar to the GM-CSF-deficient mouse, absence of alveolar macrophage PPARgamma resulted in development of lung pathology resembling PAP in 16-wk-old mice, along with excess M-CSF gene expression and secretion. In ex vivo wild-type alveolar macrophages, we observed that M-CSF itself is capable of inducing foam cell formation similar to that seen in PAP. Overexpression of PPARgamma prevented LPS-stimulated M-CSF production in RAW 264.7 cells, an effect that was abrogated by a specific PPARgamma antagonist, GW9662. Use of proteasome inhibitor, MG-132 or a PPARgamma agonist, pioglitazone, prevented LPS-mediated M-CSF induction. Using chromatin immunoprecipitation, we found that PPARgamma is capable of regulating M-CSF through transrepression of NF-kappaB binding at the promoter. Gel-shift assay experiments confirmed that pioglitazone is capable of blocking NF-kappaB binding. Taken together, these data suggest that M-CSF is an important mediator of alveolar macrophage homeostasis, and that transcriptional control of M-CSF production is regulated by NF-kappaB and PPARgamma.
Links	PubMed PMC2819287
Keywords	Animals; Cells, Cultured; Gene Expression Regulation; Humans; Macrophage Colony-Stimulating Factor/deficiency; Macrophage Colony-Stimulating Factor/genetics; Macrophage Colony-Stimulating Factor/metabolism; Macrophages, Alveolar/drug effects; Macrophages, Alveolar/metabolism; Mice; Mice, Knockout; NF-kappa B/metabolism; PPAR gamma/deficiency; PPAR gamma/genetics; PPAR gamma/metabolism; Phenotype; Promoter Regions, Genetic/genetics; Protein Binding; Response Elements; Thiazolidinediones/pharmacology
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