PMID:18503751

Citation	Ohbayashi, N, Taira, N, Kawakami, S, Togi, S, Sato, N, Ikeda, O, Kamitani, S, Muromoto, R, Sekine, Y and Matsuda, T (2008) An RNA biding protein, Y14 interacts with and modulates STAT3 activation. Biochem. Biophys. Res. Commun. 372:475-9
Abstract	Signal transducer and activator of transcription 3 (STAT3), which mediates biological actions in many physiological processes, is activated by cytokines and growth factors via specific tyrosine-phosphorylation, dimerization, and nuclear translocation. To clarify the molecular mechanisms underlying the regulation of STAT3 activation, we performed yeast two-hybrid screening. We identified Y14, an RNA-binding protein, as a novel STAT3 binding partner. Y14 bound to STAT3 through the C-terminal region of STAT3 in vivo. Importantly, small-interfering RNA-mediated reduction of endogenous Y14 expression decreased IL-6-induced tyrosine-phosphorylation, nuclear accumulation, and DNA-binding activity of STAT3, as well as IL-6/STAT3-dependent gene expression. These results indicate that Y14 interacts with STAT3 and regulates the transcriptional activation of STAT3 by influencing the tyrosine-phosphorylation of STAT3.
Links	PubMed Online version:10.1016/j.bbrc.2008.05.073
Keywords	Cell Nucleus/metabolism; DNA/metabolism; Gene Expression Regulation; Humans; Interleukin-6/metabolism; Phosphorylation; RNA, Small Interfering/genetics; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; STAT3 Transcription Factor/agonists; STAT3 Transcription Factor/genetics; STAT3 Transcription Factor/metabolism; Transcription, Genetic; Two-Hybrid System Techniques; Tyrosine/metabolism
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