PMID:18425118

Citation	Kinchen, JM, Doukoumetzidis, K, Almendinger, J, Stergiou, L, Tosello-Trampont, A, Sifri, CD, Hengartner, MO and Ravichandran, KS (2008) A pathway for phagosome maturation during engulfment of apoptotic cells. Nat. Cell Biol. 10:556-66
Abstract	Removal of apoptotic cells is critical for the physiological well-being of the organism and defects in corpse removal have been linked to disease states. Genes regulating corpse recognition and internalization have been identified, but few molecules involved in the processing of internalized corpses are known. Through a combination of targeted and unbiased reverse genetic screens in Caenorhabditis elegans, and studies in mammalian cells, we have identified genes required for maturation of apoptotic-cell-containing phagosomes. We have further ordered these candidates, which include the GTPases RAB-5 and RAB-7 and the HOPS complex, into a coherent linear pathway for the maturation of apoptotic cells within phagosomes. In depth analysis of two additional candidate genes, the phosphatidylinositol 3 kinase (PI(3)K) vps-34 (A001762) and dyn-1/dynamin, showed an accumulation of internalized, but undegraded, corpses within abnormal Rab5-negative phagosomes. We ordered these candidates in our pathway, with DYN-1 functioning upstream of VPS-34 in the recruitment and/or retention of RAB-5 to the phagosome. Finally, we have also identified a previously undescribed biochemical complex containing Vps34, dynamin and Rab5(GDP), thus providing a mechanism for Rab5 recruitment to the nascent phagosome.
Links	PubMed PMC2851549 Online version:10.1038/ncb1718
Keywords	Animals; Animals, Genetically Modified; Apoptosis/physiology; Caenorhabditis elegans/cytology; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Dynamins/genetics; Dynamins/metabolism; Humans; Multiprotein Complexes/genetics; Multiprotein Complexes/metabolism; Phagocytosis/physiology; Phagosomes/metabolism; Phosphatidylinositol 3-Kinases/genetics; Phosphatidylinositol 3-Kinases/metabolism; RNA Interference; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; rab GTP-Binding Proteins/genetics; rab GTP-Binding Proteins/metabolism; rab5 GTP-Binding Proteins/genetics; rab5 GTP-Binding Proteins/metabolism
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