GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:18423437
| Citation |
Wang, H, Ding, T, Brown, N, Yamamoto, Y, Prince, LS, Reese, J and Paria, BC (2008) Zonula occludens-1 (ZO-1) is involved in morula to blastocyst transformation in the mouse. Dev. Biol. 318:112-25 |
|---|---|
| Abstract |
It is unknown whether or not tight junction formation plays any role in morula to blastocyst transformation that is associated with development of polarized trophoblast cells and fluid accumulation. Tight junctions are a hallmark of polarized epithelial cells and zonula occludens-1 (ZO-1) is a known key regulator of tight junction formation. Here we show that ZO-1 protein is first expressed during compaction of 8-cell embryos. This stage-specific appearance of ZO-1 suggests its participation in morula to blastocyst transition. Consistent with this idea, we demonstrate that ZO-1 siRNA delivery inside the blastomeres of zona-weakened embryos using electroporation not only knocks down ZO-1 gene and protein expressions, but also inhibits morula to blastocyst transformation in a concentration-dependent manner. In addition, ZO-1 inactivation reduced the expression of Cdx2 and Oct-4, but not ZO-2 and F-actin. These results provide the first evidence that ZO-1 is involved in blastocyst formation from the morula by regulating accumulation of fluid and differentiation of nonpolar blastomeres to polar trophoblast cells. |
| Links |
PubMed PMC2442465 Online version:10.1016/j.ydbio.2008.03.008 |
| Keywords |
Actins/metabolism; Animals; Blastocyst/cytology; Blastocyst/metabolism; Cadherins/metabolism; Cell Differentiation/physiology; Electroporation; Female; Gene Expression Regulation, Developmental; Homeodomain Proteins/genetics; Homeodomain Proteins/metabolism; Male; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mice; Morula/cytology; Morula/metabolism; Octamer Transcription Factor-3/metabolism; Phosphoproteins/genetics; Phosphoproteins/metabolism; Pregnancy; RNA, Small Interfering/genetics; RNA, Small Interfering/metabolism; Tight Junctions/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
