GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:18406325
Citation |
Abdelhakim, AH, Oakes, EC, Sauer, RT and Baker, TA (2008) Unique contacts direct high-priority recognition of the tetrameric Mu transposase-DNA complex by the AAA+ unfoldase ClpX. Mol. Cell 30:39-50 |
---|---|
Abstract |
Clp/Hsp100 ATPases remodel and disassemble multiprotein complexes, yet little is known about how they preferentially recognize these complexes rather than their constituent subunits. We explore how substrate multimerization modulates recognition by the ClpX unfoldase using a natural substrate, MuA transposase. MuA is initially monomeric but forms a stable tetramer when bound to transposon DNA. Destabilizing this tetramer by ClpX promotes an essential transition in the phage Mu recombination pathway. We show that ClpX interacts more tightly with tetrameric than with monomeric MuA. Residues exposed only in the MuA tetramer are important for enhanced recognition--which requires the N domain of ClpX--as well as for a high maximal disassembly rate. We conclude that an extended set of potential enzyme contacts are exposed upon assembly of the tetramer and function as internal guides to recruit ClpX, thereby ensuring that the tetrameric complex is a high-priority substrate. |
Links |
PubMed PMC2717000 Online version:10.1016/j.molcel.2008.02.013 |
Keywords |
ATPases Associated with Diverse Cellular Activities; Adenosine Triphosphatases/chemistry; Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Amino Acid Sequence; Amino Acid Substitution; Binding Sites; DNA/chemistry; DNA/metabolism; Endopeptidase Clp/chemistry; Endopeptidase Clp/genetics; Endopeptidase Clp/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Macromolecular Substances/chemistry; Macromolecular Substances/metabolism; Models, Molecular; Molecular Chaperones/chemistry; Molecular Chaperones/genetics; Molecular Chaperones/metabolism; Point Mutation; Protein Binding; Protein Structure, Quaternary; Transposases/chemistry; Transposases/genetics; Transposases/metabolism |
Significance
Annotations
Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|
part_of |
GO:0018995: host |
ECO:0000315: mutant phenotype evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
part_of |
GO:0018995: host |
ECO:0000315: mutant phenotype evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
Notes
See also
References
See Help:References for how to manage references in GONUTS.