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PMID:18400104
| Citation |
Kunarso, G, Wong, KY, Stanton, LW and Lipovich, L (2008) Detailed characterization of the mouse embryonic stem cell transcriptome reveals novel genes and intergenic splicing associated with pluripotency. BMC Genomics 9:155 |
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| Abstract |
Transcriptional control of embryonic stem (ES) cell pluripotency has been a subject of intense study. Transcriptional regulators including Oct4 (Oct3/4 index), Sox2 and Nanog are fundamental for maintaining the undifferentiated state. However, the ES cell transcriptome is not limited to their targets, and exhibits considerable complexity when assayed with microarray, MPSS, cDNA/EST sequencing, and SAGE technologies. To identify novel genes associated with pluripotency, we globally searched for ES transcripts not corresponding to known genes, validated their sequences, determined their expression profiles, and employed RNAi to test their function. |
| Links |
PubMed PMC2375908 Online version:10.1186/1471-2164-9-155 |
| Keywords |
Animals; Embryonic Stem Cells/metabolism; Gene Expression Profiling; Mice; Pluripotent Stem Cells/metabolism; RNA Interference; RNA Splicing; Sequence Analysis, DNA; Transcription Factors/metabolism; Transcription, Genetic |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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See also
References
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