GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:18394993
| Citation |
Dawlaty, MM, Malureanu, L, Jeganathan, KB, Kao, E, Sustmann, C, Tahk, S, Shuai, K, Grosschedl, R and van Deursen, JM (2008) Resolution of sister centromeres requires RanBP2-mediated SUMOylation of topoisomerase IIalpha. Cell 133:103-15 |
|---|---|
| Abstract |
RanBP2 is a nucleoporin with SUMO E3 ligase activity that functions in both nucleocytoplasmic transport and mitosis. However, the biological relevance of RanBP2 and the in vivo targets of its E3 ligase activity are unknown. Here we show that animals with low amounts of RanBP2 develop severe aneuploidy in the absence of overt transport defects. The main chromosome segregation defect in cells from these mice is anaphase-bridge formation. Topoisomerase IIalpha (Topo IIalpha), which decatenates sister centromeres prior to anaphase onset to prevent bridges, fails to accumulate at inner centromeres when RanBP2 levels are low. We find that RanBP2 sumoylates Topo IIalpha in mitosis and that this modification is required for its proper localization to inner centromeres. Furthermore, mice with low amounts of RanBP2 are highly sensitive to tumor formation. Together, these data identify RanBP2 as a chromosomal instability gene that regulates Topo IIalpha by sumoylation and suppresses tumorigenesis. |
| Links |
PubMed PMC2693193 Online version:10.1016/j.cell.2008.01.045 |
| Keywords |
Anaphase; Aneuploidy; Animals; Antigens, Neoplasm/metabolism; Carcinogens; Centromere/metabolism; DNA Topoisomerases, Type II/metabolism; DNA-Binding Proteins/metabolism; Fibroblasts/cytology; Fibroblasts/metabolism; Mice; Mice, Knockout; Mitosis; Molecular Chaperones/genetics; Molecular Chaperones/metabolism; Mutation; Neoplasms/chemically induced; Neoplasms/metabolism; Nuclear Pore Complex Proteins/genetics; Nuclear Pore Complex Proteins/metabolism; Protein Structure, Tertiary; Small Ubiquitin-Related Modifier Proteins/metabolism; Ubiquitin-Protein Ligases/chemistry; Ubiquitin-Protein Ligases/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
