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PMID:18344988
| Citation |
Sanchez-Diaz, A, Marchesi, V, Murray, S, Jones, R, Pereira, G, Edmondson, R, Allen, T and Labib, K (2008) Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast. Nat. Cell Biol. 10:395-406 |
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| Abstract |
By rapidly depleting each of the essential budding yeast proteins of unknown function, we identified a novel factor that we call Inn1, which associates with the contractile actomyosin ring at the end of mitosis and is needed for cytokinesis. We show that Inn1 has a C2 domain at the amino terminus of the protein that is required for ingression of the plasma membrane, whereas the remainder of the protein recruits Inn1 to the actomyosin ring. The lethal effects of deleting the INN1 gene can be suppressed by artificial fusion of the C2 domain to other components of the actomyosin ring, restoring membrane ingression on contraction of the actomyosin ring. Our data indicate that recruitment of the C2 domain of Inn1 to the contractile actomyosin ring is crucial for ingression of the plasma membrane during cytokinesis in budding yeast. |
| Links |
PubMed Online version:10.1038/ncb1701 |
| Keywords |
Actomyosin/metabolism; Amino Acid Sequence; Cell Cycle Proteins/chemistry; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cell Membrane/metabolism; Cell Membrane/ultrastructure; Cytokinesis/physiology; Cytoskeletal Proteins/chemistry; Cytoskeletal Proteins/genetics; Cytoskeletal Proteins/metabolism; Cytoskeleton/metabolism; Cytoskeleton/ultrastructure; Mitosis/physiology; Models, Molecular; Molecular Sequence Data; Protein Conformation; Proteomics; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae/ultrastructure; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Sequence Alignment |
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