PMID:18298445

Citation	Rokney, A, Kobiler, O, Amir, A, Court, DL, Stavans, J, Adhya, S and Oppenheim, AB (2008) Host responses influence on the induction of lambda prophage. Mol. Microbiol. 68:29-36
Abstract	Inactivation of bacteriophage lambda CI repressor leads almost exclusively to lytic development. Prophage induction can be initiated either by DNA damage or by heat treatment of a temperature-sensitive repressor. These two treatments also cause a concurrent activation of either the host SOS or heat-shock stress responses respectively. We studied the effects of these two methods of induction on the lytic pathway by monitoring the activation of different lambda promoters, and found that the lambda genetic network co-ordinates information from the host stress response networks. Our results show that the function of the CII transcriptional activator, which facilitates the lysogenic developmental pathway, is not observed following either method of induction. Mutations in the cro gene restore the CII function irrespective of the induction method. Deletion of the heat-shock protease gene ftsH can also restore CII function following heat induction but not following SOS induction. Our findings highlight the importance of the elimination of CII function during induction as a way to ensure an efficient lytic outcome. We also show that, despite the common inhibitory effect on CII function, there are significant differences in the heat- and SOS-induced pathways leading to the lytic cascade.
Links	PubMed PMC2327240 Online version:10.1111/j.1365-2958.2008.06119.x
Keywords	Bacteriophage lambda/drug effects; Bacteriophage lambda/genetics; Bacteriophage lambda/radiation effects; DNA-Binding Proteins/genetics; DNA-Binding Proteins/physiology; Lysogeny/drug effects; Lysogeny/genetics; Lysogeny/radiation effects; Mitomycin/pharmacology; Promoter Regions, Genetic/genetics; Repressor Proteins/genetics; Repressor Proteins/physiology; SOS Response (Genetics)/genetics; Temperature; Transcription Factors/genetics; Transcription Factors/physiology; Ultraviolet Rays; Viral Proteins/genetics; Viral Proteins/physiology; Viral Regulatory and Accessory Proteins/genetics; Viral Regulatory and Accessory Proteins/physiology; Virus Activation/drug effects; Virus Activation/genetics; Virus Activation/radiation effects
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
LAMBD:RPC2		GO:0016563: transcription activator activity	ECO:0000314:		F		figure 2B shows the up regulation of CII as a heat shock response, which occurs so that transcription of CI can be activated and the cell can enter the lysogeny pathway	complete CACAO 9959

References

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