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PMID:18281296
| Citation |
Omi, S, Nakata, R, Okamura-Ikeda, K, Konishi, H and Taniguchi, H (2008) Contribution of peroxisome-specific isoform of Lon protease in sorting PTS1 proteins to peroxisomes. J. Biochem. 143:649-60 |
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| Abstract |
Using an organelle proteomics approach, we previously studied the rat peroxisome in order to characterize the proteins participating in its biogenesis. A peroxisome-specific isoform of Lon (pLon) protein was accordingly identified. However, the precise role of pLon in peroxisomes remains to be elucidated. Here, we demonstrate that pLon plays a role in processing and activating a specific regulatory protein belonging to the peroxisome targeting signal (PTS) 1-containing proteins. Proteomic analysis of proteins co-immunoprecipitated with Lon suggested that Lon interacts with PMP70 and several enzymes involved in beta-oxidation, including acyl-CoA oxidase (AOX). The processing of AOX for its activation in peroxisomes was strongly inhibited in cells expressing a dominant negative form of pLon. Furthermore, a catalase possessing a modified PTS1 sequence was misdistributed in this cell line. pLon exhibits little, if any, in vitro AOX processing activity, and does not process PTS2-containing 3-ketoacyl-coenzyme A thiolase (PTL). Therefore, pLon may specifically control, sort and process PTS1 proteins. Based on the relationship between pLon and the beta-oxidation enzymes that regulate peroxisomal morphology, the observation of enlarged peroxisomes in cells expressing recombinant pLon suggests that pLon is a critical factor determining peroxisome morphology. |
| Links |
PubMed Online version:10.1093/jb/mvn020 |
| Keywords |
Acyl-CoA Oxidase/metabolism; Catalase/analysis; Cell Line; Enzymes/metabolism; Humans; Peroxisomes/enzymology; Peroxisomes/ultrastructure; Protease La/chemistry; Protease La/metabolism; Protein Isoforms/chemistry; Protein Isoforms/metabolism; Protein Sorting Signals; Protein Transport; Proteins/metabolism |
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Significance
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| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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