PMID:18234838

Citation	Carneiro, T, Carvalho, C, Braga, J, Rino, J, Milligan, L, Tollervey, D and Carmo-Fonseca, M (2008) Inactivation of cleavage factor I components Rna14p and Rna15p induces sequestration of small nucleolar ribonucleoproteins at discrete sites in the nucleus. Mol. Biol. Cell 19:1499-508
Abstract	Small nucleolar RNAs (snoRNAs) associate with specific proteins forming small nucleolar ribonucleoprotein (snoRNP) particles, which are essential for ribosome biogenesis. The snoRNAs are transcribed, processed, and assembled in snoRNPs in the nucleoplasm. Mature particles are then transported to the nucleolus. In yeast, 3'-end maturation of snoRNAs involves the activity of Rnt1p endonuclease and cleavage factor IA (CFIA). We report that after inhibition of CFIA components Rna14p and Rna15p, the snoRNP proteins Nop1p, Nop58p, and Gar1p delocalize from the nucleolus and accumulate in discrete nucleoplasmic foci. The U14 snoRNA, but not U3 snoRNA, similarly redistributes from the nucleolus to the nucleoplasmic foci. Simultaneous depletion of either Rna14p or Rna15p and the nuclear exosome component Rrp6p induces accumulation of poly(A)(+) RNA at the snoRNP-containing foci. We propose that the foci detected after CFIA inactivation correspond to quality control centers in the nucleoplasm.
Links	PubMed PMC2291401 Online version:10.1091/mbc.E07-10-1015
Keywords	Base Sequence; Cell Nucleus/metabolism; Genes, Fungal; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; In Situ Hybridization, Fluorescence; Mutation; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; RNA, Fungal/genetics; RNA, Fungal/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Ribonucleoproteins, Small Nucleolar/genetics; Ribonucleoproteins, Small Nucleolar/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/antagonists & inhibitors; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; mRNA Cleavage and Polyadenylation Factors/antagonists & inhibitors; mRNA Cleavage and Polyadenylation Factors/genetics; mRNA Cleavage and Polyadenylation Factors/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
YEAS8:C8Z4Q5		GO:0000154: rRNA processing	ECO:0000314:		P		See Figure 1 for immunofluorescence microscopy results	complete CACAO 5052
YEAS8:C8Z4Q5		GO:0000154: rRNA modification	ECO:0000314:		P		See Figure 1 for immunofluorescence microscopy results.	complete CACAO 5184

References

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