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Neupärtl, M, Meyer, C, Woll, I, Frohns, F, Kang, M, Van Etten, JL, Kramer, D, Hertel, B, Moroni, A and Thiel, G (2008) Chlorella viruses evoke a rapid release of K+ from host cells during the early phase of infection. Virology 372:340-8
Infection of Chlorella NC64A cells by PBCV-1 produces a rapid depolarization of the host probably by incorporation of a viral-encoded K(+) channel (Kcv) into the host membrane. To examine the effect of an elevated conductance, we monitored the virus-stimulated efflux of K(+) from the chlorella cells. The results indicate that all 8 chlorella viruses tested evoked a host specific K(+) efflux with a concomitant decrease in the intracellular K(+). This K(+) efflux is partially reduced by blockers of the Kcv channel. Qualitatively these results support the hypothesis that depolarization and K(+) efflux are at least partially mediated by Kcv. The virus-triggered K(+) efflux occurs in the same time frame as host cell wall degradation and ejection of viral DNA. Therefore, it is reasonable to postulate that loss of K(+) and associated water fluxes from the host lower the pressure barrier to aid ejection of DNA from the virus particles into the host.
Barium/pharmacology; Cesium/pharmacology; Chlorella/drug effects; Chlorella/metabolism; Chlorella/virology; Gene Expression Regulation, Viral/physiology; Phycodnaviridae/genetics; Phycodnaviridae/physiology; Potassium/metabolism; Potassium Channel Blockers/pharmacology; Viral Proteins/genetics; Viral Proteins/metabolism
|Gene product||Qualifier||GO Term||Evidence Code||with/from||Aspect||Extension||Notes||Status|
|GO:0006813: potassium ion transport||
During early infection, Chorella evoke an increase in [K+]. The mechanism for this efflux was hypothesized to be Kcv proteins.As the author used a Kcv blockers like Ba2+ and Cs+ and the usage of them resulted in decrease in potassium efflux (Fig. 5). The Ba2+ inhibition of virus-triggered K+ efflux is also reflected in a reduced release of K+ from the cells (Fig. 5B). Thus Kcv is involved in the potassium ion transport.
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