PMID:18029348

Citation	Barbe, L, Lundberg, E, Oksvold, P, Stenius, A, Lewin, E, Björling, E, Asplund, A, Pontén, F, Brismar, H, Uhlén, M and Andersson-Svahn, H (2008) Toward a confocal subcellular atlas of the human proteome. Mol. Cell Proteomics 7:499-508
Abstract	Information on protein localization on the subcellular level is important to map and characterize the proteome and to better understand cellular functions of proteins. Here we report on a pilot study of 466 proteins in three human cell lines aimed to allow large scale confocal microscopy analysis using protein-specific antibodies. Approximately 3000 high resolution images were generated, and more than 80% of the analyzed proteins could be classified in one or multiple subcellular compartment(s). The localizations of the proteins showed, in many cases, good agreement with the Gene Ontology localization prediction model. This is the first large scale antibody-based study to localize proteins into subcellular compartments using antibodies and confocal microscopy. The results suggest that this approach might be a valuable tool in conjunction with predictive models for protein localization.
Links	PubMed Online version:10.1074/mcp.M700325-MCP200
Keywords	Antibodies, Neoplasm; Cell Compartmentation; Cell Line, Tumor; Humans; Internet; Microscopy, Confocal; Protein Transport; Proteome/metabolism; Subcellular Fractions
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:EGFR_original		GO:0005737: cytoplasm	IDA: Inferred from Direct Assay: :	C
HUMAN:EGFR_original		GO:0005886: plasma membrane	IDA: Inferred from Direct Assay: :	C
HUMAN:EGFR_original		GO:0005737: cytoplasm	IDA: Inferred from Direct Assay:	C
HUMAN:EGFR_original		GO:0005886: plasma membrane	IDA: Inferred from Direct Assay:	C

References

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