GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:18003741
| Citation |
Neves, A, English, K and Priess, JR (2007) Notch-GATA synergy promotes endoderm-specific expression of ref-1 in C. elegans. Development 134:4459-68 |
|---|---|
| Abstract |
The Notch signaling pathway is involved in a wide variety of cell-fate decisions during development. The diverse behavior of Notch-activated cells is thought to depend on tissue- or cell-type-specific transcription factors, yet the identities of such factors and the mechanism of cooperation with the Notch pathway are largely unknown. We identify here an enhancer in the promoter of ref-1, a C. elegans Notch target, which promotes Notch-dependent expression in mesodermal and endodermal cells. The enhancer contains predicted binding sites for the Notch transcriptional effector LAG-1/CSL that are essential for expression, a non-CSL site required for mesodermal expression, and four predicted binding sites for GATA transcription factors that are required for endodermal expression. We show that endodermal expression involves the GATA transcription factor ELT-2, and that ELT-2 can bind LAG-1/CSL in vitro. In many types of Notch-activated embryonic cells, ectopic ELT-2 is sufficient to drive expression of reporters containing the enhancer. |
| Links |
PubMed Online version:10.1242/dev.008680 |
| Keywords |
Animals; Animals, Genetically Modified; Base Sequence; Basic Helix-Loop-Helix Transcription Factors/genetics; Basic Helix-Loop-Helix Transcription Factors/metabolism; Caenorhabditis elegans/embryology; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; DNA Probes/genetics; DNA, Helminth/genetics; Endoderm/embryology; Endoderm/metabolism; Enhancer Elements, Genetic; GATA Transcription Factors/genetics; GATA Transcription Factors/metabolism; Gene Expression Regulation, Developmental; Genes, Helminth; Green Fluorescent Proteins/genetics; Molecular Sequence Data; Promoter Regions, Genetic; Receptors, Notch/genetics; Receptors, Notch/metabolism; Recombinant Proteins/genetics; Sequence Homology, Nucleic Acid; Signal Transduction |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
See also
References
See Help:References for how to manage references in GONUTS.
