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PMID:17944809
| Citation |
Pérez-Pérez, JM, Serralbo, O, Vanstraelen, M, González, C, Criqui, MC, Genschik, P, Kondorosi, E and Scheres, B (2008) Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C). Plant J. 53:78-89 |
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| Abstract |
To investigate the specialization of the two Arabidopsis CDC27 subunits in the anaphase-promoting complex (APC/C), we analyzed novel alleles of HBT/CDC27B and CDC27A, and characterized the expression of complementing HOBBIT (HBT) protein fusions in plant meristems and during the cell cycle. In contrast to other APC/C mutants, which are gametophytic lethal, phenotypes of weak and null hbt alleles indicate a primary role in the control of post-embryonic cell division and cell elongation, whereas cdc27a nulls are phenotypically indistinguishable from the wild type. However, cdc27a hbt double-mutant gametes are non-viable, indicating a redundant requirement for both CDC27 subunits during gametogenesis. Yeast-two-hybrid and pulldown studies with APC/C components suggest that the two Arabidopsis CDC27 subunits participate in several complexes that are differentially required during plant development. Loss-of-function analysis, as well as cyclin B reporter protein accumulation, indicates a conserved role for the plant APC/C in controlling mitotic progression and cell differentiation during the entire life cycle. |
| Links |
PubMed Online version:10.1111/j.1365-313X.2007.03312.x |
| Keywords |
Alleles; Arabidopsis/embryology; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Arabidopsis Proteins/physiology; Cell Cycle Proteins/chemistry; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; DNA Polymerase III/chemistry; DNA Polymerase III/genetics; DNA Polymerase III/metabolism; Genes, Plant; Mitosis/physiology; Protein Subunits; Schizosaccharomyces pombe Proteins/chemistry; Schizosaccharomyces pombe Proteins/genetics; Schizosaccharomyces pombe Proteins/metabolism; Ubiquitin-Protein Ligase Complexes/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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